Comparison of a new, affordable flow cytometric method and the manual magnetic bead technique for CD4 T-lymphocyte counting in a northern Nigerian setting

被引：21

作者：

Imade, GE

Badung, B

Pam, S

Agbaji, O

Egah, D

Sagay, AS

Sankalé, JL

Kapiga, S

Idoko, J

Kanki, P

机构：

[1] Univ Jos, Teaching Hosp, AIDS Prevent Initiat Nigeria, Jos, Nigeria

[2] Harvard Univ, Sch Publ Hlth, Boston, MA 02115 USA

来源：

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY | 2005年 / 12卷 / 01期

关键词：

D O I：

10.1128/CDLI.12.1.224-227.2005

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We compared two techniques for CD4 T-lymphocyte counting: flow cytometry (Cyflow) and magnetic beads (Dynabead). Similar results with good correlation were obtained from the 40 adult blood samples counted (P = 0.057, r = 0.93). The Cyflow technique is more precise and cost-effective than the Dynabead method ($3 to $5 versus $12 to $22 per test, respectively), since as many as 200 samples can be measured per day.

引用

收藏

页码：224 / 227

页数：4

相关论文

共 13 条

[1]

[Anonymous], AIDS EP UPD

[2] Prices of CD4 assays and viral load tests must be reduced for developing countries [J].

Bartholomew, C .

BMJ-BRITISH MEDICAL JOURNAL, 2001, 323 (7316) :809-810

[3] A MANUAL BEAD ASSAY FOR THE DETERMINATION OF ABSOLUTE CD4(+) AND CD8(+) LYMPHOCYTE COUNTS IN HUMAN IMMUNODEFICIENCY VIRUS-INFECTED INDIVIDUALS [J].

CARELLA, AV ;

MOSS, MW ;

PROVOST, V ;

QUINN, TC .

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 1995, 2 (05) :623-625

[4] Monitoring of human immunodeficiency virus infection in resource-constrained countries [J].

Crowe, S ;

Turnbull, S ;

Oelrichs, R ;

Dunne, A .

CLINICAL INFECTIOUS DISEASES, 2003, 37 :S25-S35

[5] Successful implementation of a low-cost method for enumerating CD4+ T lymphocytes in resource-limited settings:: the ANRS 12-26 study [J].

Diagbouga, S ;

Chazallon, C ;

Kazatchkine, MD ;

de Perre, PV ;

Inwoley, A ;

M'Boup, S ;

David, MP ;

Ténin, AT ;

Soudré, R ;

Aboulker, JP ;

Weiss, L .

AIDS, 2003, 17 (15) :2201-2208

[6] Individual patient-dependent influence of erythrocyte lysing procedures on flow-cytometric analysis of leukocyte subpopulations [J].

Göhde, W ;

Cassens, U ;

Lehman, LG ;

Traoré, Y ;

Göhde, W ;

Berkes, P ;

Westerberg, C ;

Greve, B .

TRANSFUSION MEDICINE AND HEMOTHERAPY, 2003, 30 (04) :165-170

[7] High-grade loss of leukocytes and hematopoietic progenitor cells caused by erythrocyte-lysing procedures for flow cytometric analyses [J].

Greve, B ;

Beller, C ;

Cassens, U ;

Sibrowski, W ;

Severin, E ;

Göhde, W .

JOURNAL OF HEMATOTHERAPY & STEM CELL RESEARCH, 2003, 12 (03) :321-330

[8] A new no-lyse, no-wash flow-cytometric method for the determination of CD4 T cells in blood samples [J].

Greve, B ;

Cassens, U ;

Westerberg, C ;

Jun, WG ;

Sibrowski, W ;

Reichelt, D ;

Göhde, W .

TRANSFUSION MEDICINE AND HEMOTHERAPY, 2003, 30 (01) :8-13

[9] Affordable CD4+-T-Cell counting by flow cytometry:: CD45 Gating for volumetric analysis [J].

Janossy, G ;

Jani, IV ;

Bradley, NJ ;

Bikoue, A ;

Pitfield, T ;

Glencross, DK .

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 2002, 9 (05) :1085-1094

[10] Affordable CD4+ T-cell counts on 'single-platform' flow cytometers I.: Primary CD4 gating [J].

Janossy, G ;

Jani, I ;

Göhde, W .

BRITISH JOURNAL OF HAEMATOLOGY, 2000, 111 (04) :1198-1208