A novel hierarchical nanozeolite composite as sorbent for protein separation in immobilized metal-ion affinity chromatography

被引:71
作者
Xu, F [1 ]
Wang, YJ [1 ]
Wang, XD [1 ]
Zhang, YH [1 ]
Tang, Y [1 ]
Yang, PY [1 ]
机构
[1] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China
关键词
D O I
10.1002/adma.200305287
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A new hierarchical porous zeolite-diatomite composite (see Figure inset) of excellent mechanical and chemical stability, after immobilization of Co2+ by an ion-exchange process, was utilized as a packing sorbent for the separation of peptides and proteins containing histidine clusters. The Figure shows the chromatographic profile for two peptides separated-using immobilized metal-ion affinity chromatography.
引用
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页码:1751 / +
页数:4
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