Regulatory elements of the floral homeotic gene AGAMOUS identified by phylogenetic footprinting and shadowing

被引:186
作者
Hong, RL
Hamaguchi, L
Busch, MA
Weigel, D [1 ]
机构
[1] Salk Inst Biol Studies, Plant Biol Lab, La Jolla, CA 92037 USA
[2] Univ Calif San Diego, Dept Biol, La Jolla, CA 92093 USA
[3] Max Planck Inst Dev Biol, Dept Mol Biol, D-72076 Tubingen, Germany
关键词
D O I
10.1105/tpc.009548
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In Arabidopsis thaliana, cis-regulatory sequences of the floral homeotic gene AGAMOUS (AG) are located in the second intron. This 3-kb intron contains binding sites for two direct activators of AG, LEAFY (LFY) and WUSCHEL (WUS), along with other putative regulatory elements. We have used phylogenetic footprinting and the related technique of phylogenetic shadowing to identify putative cis-regulatory elements in this intron. Among 29 Brassicaceae species, several other motifs, but not the LFY and WUS binding sites identified previously, are largely invariant. Using reporter gene analyses, we tested six of these motifs and found that they are all functionally important for the activity of AG regulatory sequences in A. thaliana. Although there is little obvious sequence similarity outside the Brassicaceae, the intron from cucumber AG has at least partial activity in A. thaliana. Our studies underscore the value of the comparative approach as a tool that complements gene-by-gene promoter dissection but also demonstrate that sequence-based studies alone are insufficient for a complete identification of cis-regulatory sites.
引用
收藏
页码:1296 / 1309
页数:14
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