MiR-29a Inhibits Cell Proliferation and Induces Cell Cycle Arrest through the Downregulation of p42.3 in Human Gastric Cancer

被引：90

作者：

Cui, Yun ^{[1
]}

Su, Wen-Yu ^{[1
]}

Xing, Jing ^{[1
]}

Wang, Ying-Chao ^{[1
]}

Wang, Ping ^{[2
]}

Chen, Xiao-Yu ^{[1
]}

Shen, Zhi-Yong ^{[3
]}

Cao, Hui ^{[3
]}

Lu, You-Yong ^{[4
]}

Fang, Jing-Yuan ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Div Gastroenterol & Hepatol, Renji Hosp, Sch Med,Shanghai Inst Digest Dis, Shanghai 200030, Peoples R China

[2] Shanghai Jiao Tong Univ, GI Div, Peoples Hosp 9, Sch Med, Shanghai 200030, Peoples R China

[3] Shanghai Jiao Tong Univ, GI Surg Div, Sch Med, Renji Hosp, Shanghai 200030, Peoples R China

[4] Peking Univ, Mol Oncol Lab, Beijing Inst Canc Res, Sch Oncol, Beijing 100871, Peoples R China

来源：

PLOS ONE | 2011年 / 6卷 / 10期

基金：

国家高技术研究发展计划(863计划);

关键词：

D O I：

10.1371/journal.pone.0025872

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

As a newly identified and characterized gene, p42.3 is associated with cell proliferation and tumorigenicity. The expression of p42.3 is upregulated in human gastric cancer (GC), but its underlying mechanisms of action are not well understood. MicroRNAs (miRNAs) are known to play vital regulatory roles in many cellular processes. Here we utilized bioinformatics and experimental approaches to investigate the regulatory relationship between miRNAs and the p42.3 gene. We showed that miR-29a could repress p42.3 expression at both the mRNA and protein levels via directly binding to its 3'UTR. Furthermore, an inverse relationship was observed between miR-29a and p42.3 expression in gastric cancer cell lines and GC tissue samples, especially in cases where p42.3 was downregulated. Taken together, we have elucidated previously unrecognized roles of miR-29a and indicated that miR-29a may function, at least partially, by targeting the p42.3 gene in human GC.

引用

页数：8

共 34 条

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