Natural killer cell activities of synbiotic Lactobacillus casei ssp casei in conjunction with dextran

被引:51
作者
Ogawa, T [1 ]
Asai, Y [1 ]
Tamai, R [1 ]
Makimura, Y [1 ]
Sakamoto, H [1 ]
Hashikawa, S [1 ]
Yasuda, K [1 ]
机构
[1] Asahi Univ, Sch Dent, Dept Oral Microbiol, Mizuho Ku, Gifu 5010296, Japan
关键词
cytokine; dextran; Lactobacillus casei ssp; casei; natural killer; synbiotic;
D O I
10.1111/j.1365-2249.2005.02975.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
We have reported previously that Lactobacillus casei ssp. casei, together with specific substrate dextran, exhibited an adjuvant effect of stimulating humoral immune responses against bovine serum albumin (BSA) as a model antigen in BALB/c mice. In the present study, among the Lactobacillus species tested, L. casei ssp. casei with dextran significantly elevated the natural killer (NK) cell activites in spleen mononuclear cells from BALB/c mice in comparison to L. casei ssp. casei alone or other Lactobacillus species with or without dextran. Oral administration of L. casei ssp. casei together with dextran also resulted in a significant increase of NK cell activities in healthy human volunteers. Further, L. casei ssp. casei induced significant production of interleukin (IL)-12 in human peripheral blood mononuclear cells and IL-15 mRNA expression in the human intestinal epithelial cell line Caco-2. L. casei ssp. casei with dextran in food also significantly elevated the survival rate of BALB/c mice bearing Meth-A cells. Taken together, these results demonstrate that dietary synbiotic supplementation which is a combination of the L. casei ssp. casei used as a probiotic together with the dextran, a specific substrate as a prebiotic, efficiently elicits murine and human NK cell activities.
引用
收藏
页码:103 / 109
页数:7
相关论文
共 31 条
[11]   Dietary probiotic supplementation enhances natural killer cell activity in the elderly: An investigation of age-related immunological changes [J].
Gill, HS ;
Rutherfurd, KJ ;
Cross, ML .
JOURNAL OF CLINICAL IMMUNOLOGY, 2001, 21 (04) :264-271
[12]   CLONING OF A T-CELL GROWTH-FACTOR THAT INTERACTS WITH THE BETA-CHAIN OF THE INTERLEUKIN-2 RECEPTOR [J].
GRABSTEIN, KH ;
EISENMAN, J ;
SHANEBECK, K ;
RAUCH, C ;
SRINIVASAN, S ;
FUNG, V ;
BEERS, C ;
RICHARDSON, J ;
SCHOENBORN, MA ;
AHDIEH, M ;
JOHNSON, L ;
ALDERSON, MR ;
WATSON, JD ;
ANDERSON, DM ;
GIRI, JG .
SCIENCE, 1994, 264 (5161) :965-968
[13]  
Hessle C, 1999, CLIN EXP IMMUNOL, V116, P276
[14]   Effect of an oral administration of Lactobacillus casei strain Shirota on the natural killer activity of blood mononuclear cells in aged mice [J].
Hori, T ;
Kiyoshima, J ;
Yasui, H .
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2003, 67 (02) :420-422
[15]   AUGMENTATION OF MOUSE NATURAL-KILLER CELL-ACTIVITY BY LACTOBACILLUS-CASEI AND ITS SURFACE-ANTIGENS [J].
KATO, I ;
YOKOKURA, T ;
MUTAI, M .
MICROBIOLOGY AND IMMUNOLOGY, 1984, 28 (02) :209-217
[16]   Autocrine IL-15 mediates intestinal epithelial cell death via the activation of neighboring intraepithelial NK cells [J].
Kinoshita, N ;
Hiroi, T ;
Ohta, N ;
Fukuyama, S ;
Park, EJ ;
Kiyono, H .
JOURNAL OF IMMUNOLOGY, 2002, 169 (11) :6187-6192
[17]  
Lu L, 2001, AM J CLIN NUTR, V73, p1124S
[18]  
Matsuzaki T, 1996, MED MICROBIOL IMMUN, V185, P157
[19]   A probiotic strain of L-acidophilus reduces DMH-induced large intestinal tumors in male Sprague-Dawley rats [J].
McIntosh, GH ;
Royle, PJ ;
Playne, MJ .
NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, 1999, 35 (02) :153-159
[20]   Lactobacilli and streptococci induce interleukin-12 (IL-12), IL-18, and gamma interferon production in human peripheral blood mononuclear cells [J].
Miettinen, M ;
Matikainen, S ;
Vuopio-Varkila, J ;
Pirhonen, J ;
Varkila, K ;
Kurimoto, M ;
Julkunen, I .
INFECTION AND IMMUNITY, 1998, 66 (12) :6058-6062