Development of a Selective, Sensitive, and Reversible Biosensor by the Genetic Incorporation of a Metal-Binding Site into Green Fluorescent Protein

被引：58

作者：

Ayyadurai, Niraikulam ^{[1
]}

Prabhu, Nadarajan Saravanan ^{[1
]}

Deepankumar, Kanagavel ^{[1
]}

Lee, Sun-Gu ^{[2
]}

Jeong, Heon-Ho ^{[3
]}

Lee, Chang-Soo ^{[3
]}

Yun, Hyungdon ^{[1
]}

机构：

[1] Yeungnam Univ, Sch Biotechnol, Gyongsan, South Korea

[2] Pusan Natl Univ, Dept Chem Engn, Pusan, South Korea

[3] Chungnam Natl Univ, Dept Chem Engn, Taejon, South Korea

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2011年 / 50卷 / 29期

关键词：

biosensors; copper; fluorescence; genetic-code engineering; green fluorescent protein; AMINO-ACID; COPPER;

D O I：

10.1002/anie.201008289

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

There when the light goes out: A copper biosensor was created by the introduction of the metal-chelating noncanonical amino acid L-DOPA in place of L-tyrosine into green fluorescent protein by genetic-code engineering (see picture). The specific binding of Cu2+ in vitro by the modified protein was reversible and resulted in fluorescence quenching in proportion to the amount of Cu2+ present. EDTA=ethylenediaminetetraacetic acid. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：6534 / 6537

页数：4

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