A facile and efficient method for the incorporation of multiple unnatural amino acids into a single protein

被引:26
作者
Ayyadurai, Niraikulam [1 ]
Deepankumar, Kanagavel [1 ]
Prabhu, Nadarajan Saravanan [1 ]
Lee, Sungu [2 ]
Yun, Hyungdon [1 ]
机构
[1] Yeungnam Univ, Sch Biotechnol, Gyongsan, South Korea
[2] Pusan Natl Univ, Dept Chem & Biochem Engn, Pusan, South Korea
关键词
D O I
10.1039/c0cc04672h
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
One stone, two birds: Here, we have developed a simple and efficient method for the incorporation of multiple unnatural amino acids in a single protein. This single protein exhibited two different novel functionalities acquired from the genetically incorporated unnatural amino acids, which is an interesting and not an inherent property of the protein.
引用
收藏
页码:3430 / 3432
页数:3
相关论文
共 16 条
[1]   A method to site-specifically introduce methyllysine into proteins in E. coli [J].
Ai, Hui-wang ;
Lee, Jae Wook ;
Schultz, Peter G. .
CHEMICAL COMMUNICATIONS, 2010, 46 (30) :5506-5508
[2]   Site-specific incorporation of a redox-active amino acid into proteins [J].
Alfonta, L ;
Zhang, ZW ;
Uryu, S ;
Loo, JA ;
Schultz, PG .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (48) :14662-14663
[3]  
[Anonymous], 2010, ANGEW CHEM
[4]   Expansion of the genetic code enables design of a novel "gold'' class of green fluorescent proteins [J].
Bae, JH ;
Rubini, M ;
Jung, G ;
Wiegand, G ;
Seifert, MHJ ;
Azim, MK ;
Kim, JS ;
Zumbusch, A ;
Holak, TA ;
Moroder, L ;
Huber, R ;
Budisa, N .
JOURNAL OF MOLECULAR BIOLOGY, 2003, 328 (05) :1071-1081
[5]   Selective dye-labeling of newly synthesized proteins in bacterial cells [J].
Beatty, KE ;
Xie, F ;
Wang, Q ;
Tirrell, DA .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (41) :14150-14151
[6]  
BUDISA N, 2005, ENG GENETIC CODE EXP
[7]   In Vivo Double and Triple Labeling of Proteins Using Synthetic Amino Acids [J].
Lepthien, Sandra ;
Merkel, Lars ;
Budisa, Nediljko .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2010, 49 (32) :5446-5450
[8]   Reassignment of sense codons in vivo [J].
Link, AJ ;
Tirrell, DA .
METHODS, 2005, 36 (03) :291-298
[9]   Chemistry of periodate-mediated cross-linking of 3,4-dihydroxylphenylalanine-containing molecules to proteins [J].
Liu, Bo ;
Burdine, Lyle ;
Kodadek, Thomas .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (47) :15228-15235
[10]   Adding New Chemistries to the Genetic Code [J].
Liu, Chang C. ;
Schultz, Peter G. .
ANNUAL REVIEW OF BIOCHEMISTRY, VOL 79, 2010, 79 :413-444