A method to site-specifically introduce methyllysine into proteins in E. coli

被引：80

作者：

Ai, Hui-wang ^{[1
]}

Lee, Jae Wook ^{[1
]}

Schultz, Peter G. ^{[1
]}

机构：

[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

来源：

CHEMICAL COMMUNICATIONS | 2010年 / 46卷 / 30期

基金：

美国国家卫生研究院;

关键词：

RECOMBINANT PROTEINS; HISTONE METHYLATION; PYRROLYSINE ANALOG; MAMMALIAN-CELLS; CLICK CHEMISTRY; LYSINE ANALOGS;

D O I：

10.1039/c0cc00108b

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A mutant pyrrolysyl-tRNA synthetase/tRNA pair was used to genetically encode allylcarbamoyl methyllysine in bacteria. This amino acid can be converted to methyllysine with a ruthenium catalyst, providing a straightforward approach for site-specifically introducing methyllysine residues into proteins.

引用

收藏

页码：5506 / 5508

页数：3

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