Role of Endosomal Escape for Disulfide-Based Drug Delivery from Colloidal Mesoporous Silica Evaluated by Live-Cell Imaging

被引：152

作者：

Sauer, Anna M.

Schlossbauer, Axel

Ruthardt, Nadia

Cauda, Valentina

Bein, Thomas ^{[1
]}

Braeuchle, Christoph

机构：

[1] Univ Munich LMU, Dept Chem, D-81377 Munich, Germany

来源：

NANO LETTERS | 2010年 / 10卷 / 09期

关键词：

Colloidal mesoporous silica; nanoparticles; endosomal release; photosensitizer; redox-sensitive disulfide linker; drug delivery; CONTROLLED-RELEASE; PH-DRIVEN; DYNAMICS; REDOX; FUNCTIONALIZATION; MOLECULES; TRANSPORT; SYSTEM;

D O I：

10.1021/nl102180s

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Redox-driven intracellular disulfide-cleavage is a promising strategy to achieve stimuli-responsive and controlled drug release. We synthesized colloidal mesoporous silica (CMS) nanoparticles with ATTO633-labeled cysteine linked to the inner particle core via disulfide-bridges and characterized their cysteine release behavior after internalization into HuH7 cells by high-resolution fluorescence microscopy. Our study revealed that endosomal escape is a bottleneck for disulfide-linkage based drug release. Photochemical opening of the endosome leads to successful delivery of fluorescently labeled cysteine to the cytosol.

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收藏

页码：3684 / 3691

页数：8

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