Superoxide anion production by neutrophils is associated with prevalent clinical manifestations in systemic lupus erythematosus

被引：31

作者：

Alves, Celene M. O. S. ^{[2
,3
]}

Marzocchi-Machado, Cleni M. ^{[1
,4
]}

Louzada-Junior, Paulo ^{[5
]}

Azzolini, Ana Elisa C. S. ^{[1
]}

Polizello, Ana Cristina M. ^{[1
]}

de Carvalho, Ivan F. ^{[5
]}

Lucisano-Valim, Yara M. ^{[1
]}

机构：

[1] Univ Sao Paulo, Dept Quim & Fis, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Sao Paulo, Brazil

[2] Univ Sao Paulo, Dept Bioquim & Immunol, Fac Med Ribeirao Preto, BR-14040903 Sao Paulo, Brazil

[3] Univ Fed Uberlandia, Dept Imunoparasitol, Inst Ciencias Biomed, Belo Horizonte, MG, Brazil

[4] Univ Sao Paulo, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Sao Paulo, Brazil

[5] Univ Sao Paulo, Dept Clin Med, Fac Med Ribeirao Preto, BR-14040903 Sao Paulo, Brazil

来源：

CLINICAL RHEUMATOLOGY | 2008年 / 27卷 / 06期

基金：

巴西圣保罗研究基金会;

关键词：

complement receptors; FcgammaR; neutrophils; superoxide anion; systemic lupus erythematosus;

D O I：

10.1007/s10067-007-0768-x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

To determine the relation between neutrophil function and the clinical characteristics of systemic lupus erythematosus ( SLE), the superoxide anion (O-2(-)) production by neutrophils, mediated by Fc gamma R and Fc gamma R/CR cooperation, was studied in 64 SLE patients classified according to their prevalent clinical manifestations. Three clinically distinct patterns were designated: ( 1) manifestations associated with the occurrence of cytotoxic antibodies ( SLE-I group); ( 2) manifestations associated with circulating immune complexes ( IC; SLE-II group), and ( 3) manifestations associated with IC and cytotoxic antibodies ( SLE-III group). O-2(-) production was evaluated by a lucigenin-dependent chemiluminescent assay in neutrophils stimulated with ICIgG opsonized or not with complement. No difference in O-2(-) production was observed when neutrophil responses from healthy controls were compared to the unclassified patients. However, when the SLE patient groups were considered, the following differences were observed: ( 1) SLE-I neutrophils showed lower O-2(-) production mediated by the IgG receptor ( Fc gamma R) with the cooperation of complement receptors ( Fc gamma R/ CR) than observed in the SLE-II, SLE-III, and healthy groups; ( 2) neutrophils from the SLE-II group showed a decreased O-2(-) production mediated by Fc gamma R/ CR compared to the SLE-III group, ( 3) SLE-III neutrophils produced more O-2(-) than neutrophils from the SLE-II and control groups, and ( 4) CR showed inefficiency in mediating the O-2(-) production by neutrophils from the SLE-I group. Comparative experiments on the kinetics of chemiluminescence ( CL; T-max and CLmax) disclosed differences only for the SLE- I group. Taken together, these results suggest that differences in oxidative metabolism of neutrophils mediated by Fc gamma R/ CR may reflect an acquired characteristic of disease associated with distinct clinical manifestations.

引用

页码：701 / 708

页数：8

共 38 条

[31] CORRELATION OF IGG FC RECEPTORS ON GRANULOCYTES WITH SERUM IMMUNE-COMPLEX LEVEL IN SYSTEMIC LUPUS-ERYTHEMATOSUS [J].