NudE and NudEL are required for mitotic progression and are involved in dynein recruitment to kinetochores

被引：104

作者：

Stehman, Stephanie A. ^{[1
]}

Chen, Yu ^{[1
]}

McKenney, Richard J. ^{[1
]}

Vallee, Richard B. ^{[1
]}

机构：

[1] Columbia Univ, Dept Pathol & Cell Biol, New York, NY 10032 USA

来源：

JOURNAL OF CELL BIOLOGY | 2007年 / 178卷 / 04期

关键词：

D O I：

10.1083/jcb.200610112

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

NudE and l are related proteins that interact with cytoplasmic dynein and LIS1. Their functional relationship and involvement in LIS1 and dynein regulation are not completely understood. We find that NudE and l each localize to mitotic kinetochores before dynein, dynactin, ZW10, and LIS1 and exhibit additional temporal and spatial differences in distribution from the motor protein. Inhibition of NudE and l caused metaphase arrest with misoriented chromosomes and defective microtubule attachment. Dynein and dynactin were both displaced from kinetochores by the injection of an anti-NudE/NudEL antibody. Dynein but not dynactin interacted with NudE surprisingly through the dynein intermediate and light chains but not the motor domain. Together, these results identify a common function for NudE and l in mitotic progression and identify an alternative mechanism for dynein recruitment to and regulation at kinetochores.

引用

页码：583 / 594

页数：12

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