Reagentless phosphate ion sensor system for environmental monitoring

被引：10

作者：

Suzuki, M

Kurata, H

Inoue, Y

Shin, H

Kubo, I

Nakamura, H

Ikebukuro, K

Karube, I

机构：

[1] Kyushu Inst Technol, Fac Comp Sci & Syst Engn, Dept Biochem Engn & Sci, Fukuoka 8208502, Japan

[2] Soka Univ, Fac Engn, Tokyo 1928577, Japan

[3] Univ Tokyo, Adv Sci & Technol Res Ctr, Meguro Ku, Tokyo 1538904, Japan

来源：

DENKI KAGAKU | 1998年 / 66卷 / 06期

关键词：

biosensor; enzyme sensor; phosphate ion; environmental monitoring;

D O I：

10.5796/kogyobutsurikagaku.66.579

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

Phosphate ion sensor system using an electrochemical detector was developed by the use of recombinant pyruvate oxidase (PyOD) from Lactobacillus plantarum, which needs no addition of thiamine pyrophosphate and flavin adenine dinucleotide for reaction. This system could detect 2 nM hydrogen peroxide. Response time for phosphate ion was 80 s and total measurement time for one sample was 3 min. Citrate buffer solution (pH 6.3) was most suitable for the measurement and optimum flow rate was 0.6 ml/min. Under these optimum conditions, minimum detection limit of phosphate ion was 15 nM, which was enough for the determination of phosphate ion in dam-lake.

引用

页码：579 / 583

页数：5

共 6 条

[1] A novel biosensor system for the determination of phosphate [J].