Interdependence between transcription and mRNP processing and export, and its impact on genetic stability

被引：98

作者：

Luna, R ^{[1
]}

Jimeno, S ^{[1
]}

Marín, M ^{[1
]}

Huertas, P ^{[1
]}

García-Rubio, M ^{[1
]}

Aguilera, A ^{[1
]}

机构：

[1] Univ Seville, Dept Genet, Fac Biol, Seville, Spain

来源：

MOLECULAR CELL | 2005年 / 18卷 / 06期

关键词：

D O I：

10.1016/j.molcel.2005.05.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The conserved eukaryotic THO-TREX complex acts at the interface between transcription and mRNA export and affects transcription-associated recombination. To investigate the interdependence of nuclear mRNA processes and their impact on genomic integrity, we analyzed transcript accumulation and recombination of 40 selected mutants covering representative steps of the biogenesis and export of the messenger ribonucleoprotein particle (mRNP). None of the mutants analyzed shared the strong transcript-accumulation defect and hyperrecombination of THO mutants. Nevertheless, mutants in 3' end cleavage/polyadenylation, nuclear exosome, and mRNA export showed a weak but significant effect on recombination and transcript accumulation. Mutants of the nuclear exosome (rrp6) and 3' end processing factors (rna14 and rna15) showed inefficient transcription elongation and genetic interactions with THO. The results suggest a tight interdependence among mRNP biogenesis steps and transcription and an unexpected effect of the nuclear exosome and the cleavage/polyadenylation factors on transcription elongation and genetic integrity.

引用

收藏

页码：711 / 722

页数：12

相关论文

共 52 条

[1] The connection between transcription and genomic instability [J].

Aguilera, A .

EMBO JOURNAL, 2002, 21 (03) :195-201

[2] The RNA processing exosome is linked to elongating RNA polymerase II in Drosophila [J].

Andrulis, ED ;

Werner, J ;

Nazarian, A ;

Erdjument-Bromage, H ;

Tempst, P ;

Lis, JT .

NATURE, 2002, 420 (6917) :837-841

[3] Cleavage/polyadenylation factor IA associates with the carboxyl-terminal domain of RNA polymerase II in Saccharomyces cerevisiae [J].

Barillà, D ;

Lee, BA ;

Proudfoot, NJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (02) :445-450

[4] Coupling termination of transcription to messenger RNA maturation in yeast [J].

Birse, CE ;

Minvielle-Sebastia, L ;

Lee, BA ;

Keller, W ;

Proudfoot, NJ .

SCIENCE, 1998, 280 (5361) :298-301

[5] Exploring functional relationships between components of the gene expression machinery [J].

Burckin, T ;

Nagel, R ;

Mandel-Gutfreund, Y ;

Shiue, L ;

Clark, TA ;

Chong, JL ;

Chang, TH ;

Squazzo, S ;

Hartzog, G ;

Ares, M .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2005, 12 (02) :175-182

[6] The transcriptional coactivator PC4/Sub1 has multiple functions in RNA polymerase II transcription [J].

Calvo, O ;

Manley, JL .

EMBO JOURNAL, 2005, 24 (05) :1009-1020

[7] Genome-wide localization of the nuclear transport machinery couples transcriptional status and nuclear organization [J].

Casolari, JM ;

Brown, CR ;

Komili, S ;

West, J ;

Hieronymus, H ;

Silver, PA .

CELL, 2004, 117 (04) :427-439

[8] A protein complex containing Tho2, Hpr1, Mft1 and a novel protein, Thp2, connects transcription elongation with mitotic recombination in Saccharomyces cerevisiae [J].

Chávez, S ;

Beilharz, T ;

Rondón, AG ;

Erdjument-Bromage, H ;

Tempst, P ;

Svejstrup, JQ ;

Lithgow, T ;

Aguilera, A .

EMBO JOURNAL, 2000, 19 (21) :5824-5834

[9] Hpr1 is preferentially required for transcription of either long or G+C-Rich DNA sequences in Saccharomyces cerevisiae [J].

Chávez, S ;

García-Rubio, M ;

Prado, F ;

Aguilera, A .

MOLECULAR AND CELLULAR BIOLOGY, 2001, 21 (20) :7054-7064

[10] Degradation of normal mRNA in the nucleus of Saccharomyces cerevisiae [J].

Das, B ;

Butler, JS ;

Sherman, F .

MOLECULAR AND CELLULAR BIOLOGY, 2003, 23 (16) :5502-5515

← 1 2 3 4 5 6 →