Interdependence between transcription and mRNP processing and export, and its impact on genetic stability

被引：98

作者：

Luna, R ^{[1
]}

Jimeno, S ^{[1
]}

Marín, M ^{[1
]}

Huertas, P ^{[1
]}

García-Rubio, M ^{[1
]}

Aguilera, A ^{[1
]}

机构：

[1] Univ Seville, Dept Genet, Fac Biol, Seville, Spain

来源：

MOLECULAR CELL | 2005年 / 18卷 / 06期

关键词：

D O I：

10.1016/j.molcel.2005.05.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The conserved eukaryotic THO-TREX complex acts at the interface between transcription and mRNA export and affects transcription-associated recombination. To investigate the interdependence of nuclear mRNA processes and their impact on genomic integrity, we analyzed transcript accumulation and recombination of 40 selected mutants covering representative steps of the biogenesis and export of the messenger ribonucleoprotein particle (mRNP). None of the mutants analyzed shared the strong transcript-accumulation defect and hyperrecombination of THO mutants. Nevertheless, mutants in 3' end cleavage/polyadenylation, nuclear exosome, and mRNA export showed a weak but significant effect on recombination and transcript accumulation. Mutants of the nuclear exosome (rrp6) and 3' end processing factors (rna14 and rna15) showed inefficient transcription elongation and genetic interactions with THO. The results suggest a tight interdependence among mRNP biogenesis steps and transcription and an unexpected effect of the nuclear exosome and the cleavage/polyadenylation factors on transcription elongation and genetic integrity.

引用

页码：711 / 722

页数：12

共 52 条

[31] Functional interaction of yeast pre-mRNA 3′ end processing factors with RNA polymerase II [J].