Anomer-selective glucosylation of l-menthol by yeast α-glucosidase

被引：26

作者：

Nakagawa, H

Yoshiyama, M

Shimura, S

Kirimura, K

Usami, S

机构：

[1] Waseda Univ, Sch Sci & Engn, Dept Appl Chem, Shinjuku Ku, Tokyo 1698555, Japan

[2] Lotte Co Ltd, Cent Lab, Urawa, Saitama 3360027, Japan

[3] Waseda Univ, Mat Res Projects Biosci & Photon, Tokyo, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1998年 / 62卷 / 07期

关键词：

alpha-glucosidase; menthol; transglucosylation; Saccharomyces cerevisiae;

D O I：

10.1271/bbb.62.1332

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

l-Menthol was glucosylated by the alpha-glucosidase (EC 3.2.1.20) of Saccharomyces cerevisiae using maltose as the glucosyl donor. When 50 mg of l-menthol and 1.6 M maltose in 10 mM citrate-phosphate buffer (pH 5.5) were incubated at 45 degrees C, l-menthyl alpha-D-glucopyranoside (alpha-MenG) was alpha-anomer-selectively formed as a product. The specificity of the alpha-linkage was confirmed by C-13-NMR analysis. In the reaction mixture after 2 h, alpha-MenG was mainly accumulated in a crystalline form and the concentration of dissolved alpha-MenG was constant at 1.4 mM. The molar conversion yield of alpha-MenG produced based on the supplied l-menthol was maximally 30.7% at 48 h of reaction.

引用

收藏

页码：1332 / 1336

页数：5

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