A three-dimensional ultrasonic cage for characterization of individual cells

被引：60

作者：

Manneberg, Otto ^{[1
]}

Vanherberghen, Bruno ^{[1
]}

Svennebring, Jessica ^{[1
]}

Hertz, Hans M. ^{[1
]}

Onfelt, Bjorn ^{[1
,2
]}

Wiklund, Martin ^{[1
]}

机构：

[1] KTH AlbaNova, Royal Inst Technol, Dept Appl Phys, SE-10691 Stockholm, Sweden

[2] Karolinska Inst, Dept Microbiol Tumor & Cell Biol, SE-17177 Stockholm, Sweden

来源：

APPLIED PHYSICS LETTERS | 2008年 / 93卷 / 06期

基金：

瑞典研究理事会;

关键词：

D O I：

10.1063/1.2971030

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

We demonstrate enrichment, controlled aggregation, and manipulation of microparticles and cells by an ultrasonic cage integrated in a microfluidic chip compatible with high-resolution optical microscopy. The cage is designed as a dual-frequency resonant filleted square box integrated in the fluid channel. Individual particles may be trapped three dimensionally, and the dimensionality of one-dimensional to three-dimensional aggregates can be controlled. We investigate the dependence of the shape and position of a microparticle aggregate on the actuation voltages and aggregate size, and demonstrate optical monitoring of individually trapped live cells with submicrometer resolution. (C) 2008 American Institute of Physics.

引用

页数：3

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