Characterization of the Drosophila melanogaster mitochondrial proteome

被引：28

作者：

Alonso, J ^{[1
]}

Rodriguez, JM ^{[1
]}

Baena-López, LA ^{[1
]}

Santarén, JF ^{[1
]}

机构：

[1] Univ Autonoma Madrid, Ctr Biol Mol Severo Ochoa, CSIC, E-28049 Madrid, Spain

来源：

JOURNAL OF PROTEOME RESEARCH | 2005年 / 4卷 / 05期

关键词：

Drosophila; mitochondria; proteomics; 2D gels; MALDI-TOF;

D O I：

10.1021/pr050130c

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have combined high-resolution two-dimensional (2-D) gel electrophoresis with mass spectrometry with the aim of identifying proteins represented in the 2-D gel database of Drosophila melanogaster mitochondria. First, we purified mitochondria from third instar Drosophila larvae and constructed a high-resolution 2-D gel database containing 231 silver-stained polypeptides. Next, we carried out preparative 2-D PAGE to isolate some of the polypeptides and characterize them by MALDI-TOF analysis. Using this strategy, we identified 66 mitochondrial spots in the database, and in each case confirmed their identity by MALDI-TOF/TOF analysis. In addition, we generated antibodies against two of the mitochondrial proteins as tools for characterizing the organelle.

引用

页码：1636 / 1645

页数：10

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