Chromatographic fingerprint analysis and characterization of furocoumarins in the roots of Angelica dahurica by HPLC/DAD/ESI-MSn technique

被引：101

作者：

Kang, Jie ^{[1
]}

Zhou, Lei ^{[1
]}

Sun, Jianghao ^{[1
]}

Han, Jian ^{[1
]}

Guo, De-An ^{[1
]}

机构：

[1] Peking Univ, Hlth Sci Ctr, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100083, Peoples R China

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2008年 / 47卷 / 4-5期

关键词：

Angelica dahurica; furocoumarins; chromatographic fingerprint; HPLC/DAD/ESI-MSn;

D O I：

10.1016/j.jpba.2008.03.010

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学]; 081704 [应用化学];

摘要：

A high performance liquid chromatography-diode array detection-electrospray ionization tandem mass spectrometry (HPLC/DAD/ESI-MSn) method was used for the chromatographic fingerprint analysis and characterization of furocoumarins in the roots of Angelica dahurica for the first time. Nine "common peaks" were identified by comparing with the retention time, UV and MS spectra of reference furocoumarins. The software "Similarity Evaluation System for Chromatographic Fingerprint of TCM" was used to evaluate the similarities of 13 batches of Baizhi samples collected from Henan, Zhejiang, Sichuan and Anhui provinces of China. The results indicated that the samples from different batches had similar HPLC fingerprints, and the method could be applied for the quality control of the roots of Angelica dahurica. In addition, a total of 20 furocoumarins were identified or tentatively characterized by HPLC/DAD/ESI-MSn technique, and their fragmentation patterns in an electrospray ion trap mass spectrometer were also summarized. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：778 / 785

页数：8

共 38 条

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