Live single-cell molecular analysis by video-mass spectrometry

被引：59

作者：

Tsuyama, Naohiro ^{[1
]}

Mizuno, Hajime ^{[1
]}

Tokunaga, Emi ^{[1
]}

Masujima, Tsutomu ^{[1
]}

机构：

[1] Hiroshima Univ, Grad Sch Biomed Sci, Hiroshima 7348553, Japan

来源：

ANALYTICAL SCIENCES | 2008年 / 24卷 / 05期

关键词：

D O I：

10.2116/analsci.24.559

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The direct molecular analysis of a live single cell viewed under a video-microscope has been developed. The cell contents are sucked into a nano-electrospray tip, and hundreds of peaks of ionic compounds of low molecular weight are detected by nano-ESI Q-TOF mass spectrometry (MS). Cell-specific MS peaks in a single mouse-embryonic fibroblasts cell are extracted by a t-test, and one of the peaks is proceeded to MS/MS analysis for molecular identification. This method is direct and quick to identify the molecules of a cell with simultaneous observation by a video-microscope.

引用

页码：559 / 561

页数：3

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