Synthesis of [2,3,4,5,6-2H5]phenyl glucosinolate

Starting from commercially available [2,3,4,5,6-H-2(5)]benzoic acid, [2,3,4,5,6-H-2(5)]phenyl glucosinolate was synthesized. Under negative-ion electrospray-ionization mass spectrometric conditions, this compound affords a peak at m/z 399. Since this m/z value is not known from the ions derived from natural glucosinolates, the [2,3,4,5,6-H-2(6)]phenyl glucosinolate reported here is useful as an internal standard for the quantification of glucosinolates by negative-ion mass spectrometry (MS) and liquid chromatography (LC)/MS techniques. Copyright (c) 2007 John Wiley & Sons, Ltd.