Continuous production of acyl L-ascorbates using a packed-bed reactor with immobilized lipase

被引:18
作者
Kuwabara, K
Watanabe, Y
Adachi, S [1 ]
Nakanishi, K
Matsuno, R
机构
[1] Kyoto Univ, Grad Sch Agr, Div Food Sci & Biotechnol, Sakyo Ku, Kyoto 6068502, Japan
[2] Okayama Univ, Fac Engn, Dept Biosci & Biotechnol, Okayama 7008530, Japan
关键词
acyl L-ascorbate; critical micelle concentration; immobilized lipase; packed-bed reactor; surface tension;
D O I
10.1007/s11746-003-0791-0
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Saturated acyl (6-O-caproyl, lauroyl, and myristoyl) and unsaturated acyl (6-O-oleoyl, linoleoyl, and arachidonoyl) L-ascorbates were continuously synthesized at 50degreesC using a system where a column packed with ascorbic acid powder and a packed-bed reactor with an immobilized lipase from Candida antarctica were connected in series. A productivity of 1.6-1.9 kg/L reactor-cl was achieved for at least 11 d. The surface tension of the caproyl or lauroyl L-ascorbate in aqueous solution was measured at various temperatures and pH to estimate the critical micelle concentration (CMC) of the acyl L-ascorbate. The CMC values were independent of temperature but dependent on the pH. The value of the caproyl ascorbate increased with an increase in pH.
引用
收藏
页码:895 / 899
页数:5
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