Sulfated polysaccharide from the marine algae Hypnea musciformis inhibits TNBS-induced intestinal damage in rats

被引：49

作者：

Brito, Tarcisio V. ^{[1
]}

Barros, Francisco C. N. ^{[2
,5
]}

Silva, Renan O. ^{[3
]}

Dias Junior, Genilson J. ^{[1
]}

Simiao C., Jose Junior ^{[1
]}

Franco, Alvaro X. ^{[3
]}

Soares, Pedro M. G. ^{[3
,4
]}

Chaves, Luciano S. ^{[2
]}

Abreu, Clara M. W. S. ^{[6
]}

de Paula, Regina C. M. ^{[6
]}

Souza, Marcellus H. L. P. ^{[3
]}

Freitas, Ana Lucia P. ^{[2
]}

Barbosa, Andre Luiz R. ^{[1
]}

机构：

[1] Univ Fed Piaui, Biotechnol & Biodivers Ctr Res BIOTEC, LAFFEX Lab Expt Physiopharmacol, BR-64202020 Parnaiba, PI, Brazil

[2] Univ Fed Ceara, Dept Biochem & Mol Biol, Lab Prot & Carbohydrates Marine Algae, BR-60455760 Fortaleza, Ceara, Brazil

[3] Univ Fed Ceara, LEFFAG Lab Physiopharmacol Study Gastrointestinal, Fortaleza, Ceara, Brazil

[4] Univ Fed Ceara, Sch Med, Dept Morphol, Rua Delmiro de Farias S-N, BR-60416030 Fortaleza, Ceara, Brazil

[5] Fed Inst Educ Sci & Technol Ceara, Juazeiro Do Norte, CE, Brazil

[6] Univ Fed Ceara, Dept Organ & Inorgan Chem, Lab Polymers, BR-60455760 Fortaleza, Ceara, Brazil

来源：

CARBOHYDRATE POLYMERS | 2016年 / 151卷

关键词：

Sulfated polysaccharide; Marine algae; Colitis; Inflammatory disease; INFLAMMATORY-BOWEL-DISEASE; ANTICOAGULANT ACTIVITY; INDUCED COLITIS; GALACTANS; CARRAGEENANS; ANTIOXIDANT; FRACTION; AGAR;

D O I：

10.1016/j.carbpol.2016.06.047

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

Sulfated polysaccharides extracted from seaweed have important pharmacological properties. Thus, the aim of this study was to characterize the sulfated polysaccharide (PLS) from the algae Hypnea musciformis and evaluate its protective effect in colitis induced by trinitrobenzene sulfonic acid in rats. The sulfated polysaccharide possess a high molecular mass (1.24 x 10(5) g mol(-1)) and is composed of a K-carrageenan, as depicted by FT-IR and NMR spectroscopic data. PLS was administered orally (10, 30, and 60 mg/kg, p.o.) for three days, starting before TNBS (trinitrobenzene sulfonic acid) instillation (day 1). The rats were killed on day three, the portion of distal colon (5 cm) was excised and evaluated macroscopic scores and wet weight. Then, samples of the intestinal were used for histological evaluation and quantification of glutathione, malonyldialdehyde acid, myeloperoxidase, nitrate/nitrite and cytokines. Our results demonstrate that PLS reduced the colitis and all analyzed biochemical parameters. Thus, we concluded that the PLS extracted from the marine algae H. musciformis reduced the colitis in animal model and may have an important promising application in the inflammatory bowel diseases. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：957 / 964

页数：8

共 51 条

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