Isolation and in vitro expansion of human colonic stem cells

被引：569

作者：

Jung, Peter ^{[1
]}

Sato, Toshiro ^{[2
,3
,4
]}

Merlos-Suarez, Anna ^{[1
]}

Barriga, Francisco M. ^{[1
]}

Iglesias, Mar ^{[5
]}

Rossell, David ^{[6
]}

Auer, Herbert ^{[7
]}

Gallardo, Mercedes ^{[8
]}

Blasco, Maria A. ^{[8
]}

Sancho, Elena ^{[1
]}

Clevers, Hans ^{[2
,3
]}

Batlle, Eduard ^{[1
,9
]}

机构：

[1] Inst Biomed Res, Oncol Program, Barcelona, Spain

[2] Hubrecht Inst, Utrecht, Netherlands

[3] Univ Med Ctr Utrecht, Utrecht, Netherlands

[4] Keio Univ, Sch Med, Dept Gastroenterol, Shinjuku Ku, Tokyo, Japan

[5] Univ Autonoma Barcelona, Dept Pathol, Hosp Univ Mar, E-08193 Barcelona, Spain

[6] Inst Biomed Res, Biostat & Bioinformat Unit, Barcelona, Spain

[7] Inst Biomed Res, Funct Genom Core Facil, Barcelona, Spain

[8] Spanish Natl Canc Res Ctr, Telomeres & Telomerase Grp, Mol Oncol Program, Madrid, Spain

[9] Inst Catalana Recerca & Estudis Avancats, Barcelona, Spain

来源：

NATURE MEDICINE | 2011年 / 17卷 / 10期

基金：

欧洲研究理事会;

关键词：

COLORECTAL-CANCER; INTESTINAL EPITHELIUM; SELF-RENEWAL;

D O I：

10.1038/nm.2470

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Here we describe the isolation of stem cells of the human colonic epithelium. Differential cell surface abundance of ephrin type-B receptor 2 (EPHB2) allows the purification of different cell types from human colon mucosa biopsies. The highest EPHB2 surface levels correspond to epithelial colonic cells with the longest telomeres and elevated expression of intestinal stem cell (ISC) marker genes. Moreover, using culturing conditions that recreate the ISC niche, a substantial proportion of EPHB2-high cells can be expanded in vitro as an undifferentiated and multipotent population.

引用

收藏

页码：1225 / 1227

页数：3

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