Fluorescent, through-bond energy transfer cassettes for Labeling multiple biological molecules in one experiment

被引:185
作者
Jiao, GS [1 ]
Thoresen, LH [1 ]
Burgess, K [1 ]
机构
[1] Texas A&M Univ, Dept Chem, College Stn, TX 77842 USA
关键词
D O I
10.1021/ja037193l
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Through-bond energy transfer cassettes based on a fluorescein donor component electronically conjugated to rhodamine-like acceptors have been designed and synthesized. They absorb strongly at 488 nm (Ar-laser emission) and efficiently transfer the energy to the acceptor component that emits strongly. Further, the cassettes are more stable to photobleaching than fluorescein, making them potentially more suitable for single-molecule detection methods than fluorescein itself. These studies form the basis for improved detection of chain-terminated DNA in high-throughput sequencing and other applications in biotechnology. Copyright © 2003 American Chemical Society.
引用
收藏
页码:14668 / 14669
页数:2
相关论文
共 15 条
[1]   Poly(aryleneethynylene)s: Syntheses, properties, structures, and applications [J].
Bunz, UHF .
CHEMICAL REVIEWS, 2000, 100 (04) :1605-1644
[2]  
Clegg Robert M., 1995, Current Opinion in Biotechnology, V6, P103, DOI 10.1016/0958-1669(95)80016-6
[3]  
EGGELING C, 1999, APPL FLUORESC CHEM B, P192
[4]   Syntheses of regioisomerically pure 5-or 6-halogenated fluoreseeins [J].
Jiao, GS ;
Han, JW ;
Burgess, K .
JOURNAL OF ORGANIC CHEMISTRY, 2003, 68 (21) :8264-8267
[5]  
Lakowicz J.R., 2004, PRINCIPLES FLUORESCE, VSecond
[6]  
Lakowicz J.R., 1999, Principles of Fluoresence Spectroscopy, V2nd ed.
[7]   New energy transfer dyes for DNA sequencing [J].
Lee, LG ;
Spurgeon, SL ;
Heiner, CR ;
Benson, SC ;
Rosenblum, BB ;
Menchen, SM ;
Graham, RJ ;
Constantinescu, A ;
Upadhya, KG ;
Cassel, JM .
NUCLEIC ACIDS RESEARCH, 1997, 25 (14) :2816-2822
[8]  
LEE LG, 2002, Patent No. 0236832
[9]   Fluorescence resonance energy transfer terminators for DNA sequencing [J].
Nampalli, S ;
Khot, M ;
Kumar, S .
TETRAHEDRON LETTERS, 2000, 41 (46) :8867-8871
[10]   FLUORESCENCE DETECTION IN AUTOMATED DNA-SEQUENCE ANALYSIS [J].
SMITH, LM ;
SANDERS, JZ ;
KAISER, RJ ;
HUGHES, P ;
DODD, C ;
CONNELL, CR ;
HEINER, C ;
KENT, SBH ;
HOOD, LE .
NATURE, 1986, 321 (6071) :674-679