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The unusual methanogenic seryl-tRNA synthetase recognizes tRNASer species from all three kingdoms of life

被引：29
作者：
Bilokapic, S
Korencic, D
Söll, D
Weygand-Durasevic, I
机构：
[1] Univ Zagreb, Fac Sci, Dept Chem, Zagreb 10000, Croatia
[2] Rudjer Boskovic Inst, Zagreb, Croatia
[3] Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT USA
来源：
EUROPEAN JOURNAL OF BIOCHEMISTRY | 2004年 / 271卷 / 04期
关键词：
methanogenic archaea; seryl-tRNA synthetase; tRNA dimerization; tRNA(Ser) recognition;
D O I：
10.1111/j.1432-1033.2003.03971.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The methanogenic archaea Methanococcus jannaschii and M. maripaludis contain an atypical seryl-tRNA synthetase (SerRS), which recognizes eukaryotic and bacterial tRNAs(Ser), in addition to the homologous tRNA(Ser) and tRNA(Sec) species. The relative flexibility in tRNA recognition displayed by methanogenic SerRSs, shown by aminoacylation and gel mobility shift assays, indicates the conservation of some serine determinants in all three domains. The complex of M. maripaludis SerRS with the homologues tRNA(Ser) was isolated by gel filtration chromatography. Complex formation strongly depends on the conformation of tRNA. Therefore, the renaturation conditions for in vitro transcribed tRNA(GCU)(Ser) isoacceptor were studied carefully. This tRNA, unlike many other tRNAs, is prone to dimerization, possibly due to several stretches of complementary oligonucleotides within its sequence. Dimerization is facilitated by increased tRNA concentration and can be diminished by fast renaturation in the presence of 5 mM magnesium chloride.
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页码：694 / 702
页数：9
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