Synergistic effects of elastic modulus and surface topology of Ti-based implants on early osseointegration

被引:23
作者
Dai, Xiaohan [1 ,2 ]
Zhang, Xuehui [3 ]
Xu, Mingming [2 ]
Huang, Ying [2 ]
Heng, Boon Chin [4 ]
Mo, Xiaoju [2 ]
Liu, Yun [2 ]
Wei, Daqing [5 ]
Zhou, Yu [5 ]
Wei, Yan [2 ]
Deng, Xuming [1 ]
Deng, Xuliang [2 ,6 ,7 ]
机构
[1] Jilin Univ, Inst Zoonosis, Coll Vet Med, Key Lab Zoonosis,Minist Educ, Changchun 130062, Peoples R China
[2] Peking Univ, Sch & Hosp Stomatol, Dept Geriatr Dent, Beijing 100081, Peoples R China
[3] Peking Univ, Sch & Hosp Stomatol, Dept Dent Mat, Beijing 100081, Peoples R China
[4] Univ Hong Kong, Fac Dent, Pokfulam Rd, Hong Kong, Hong Kong, Peoples R China
[5] Harbin Inst Technol, Dept Mat Sci & Engn, Harbin 150080, Peoples R China
[6] Natl Engn Lab Digital & Mat Technol Stomatol, Beijing 100081, Peoples R China
[7] Peking Univ, Sch & Hosp Stomatol, Beijing Lab Biomed Mat, Beijing 100081, Peoples R China
基金
中国国家自然科学基金; 国家高技术研究发展计划(863计划);
关键词
MICROARC OXIDATION COATINGS; STEM-CELL FATE; TITANIUM SURFACE; BONE-FORMATION; FOCAL ADHESIONS; EXTRACELLULAR-MATRIX; POROUS-STRUCTURE; IN-VITRO; HYDROXYAPATITE; BEHAVIOR;
D O I
10.1039/c6ra04772f
中图分类号
O6 [化学];
学科分类号
070301 [无机化学];
摘要
Early osseointegration plays a crucial role in determining the therapeutic efficacy of orthopedic implants. Some factors responsible for early osseointegration, such as inherent mechanical properties and surface topology of implants, are well-characterized. However, the synergistic effects of elastic modulus and surface topology of implants on the osteogenic differentiation of stem cells and early osseointegration have not been thoroughly investigated. In this study, the titanium (Ti) and beta-titanium alloy Ti-24Nb4-Zr-8Sn (TNZS) were used to evaluate the synergistic effects of elastic modulus and surface topology on the biological performance of these materials in vitro and in vivo. Scanning electron microscopy imaging confirmed the presence of a micro-scale porous oxide layer on the Ti and TNZS surfaces upon treatment with microarc oxidation (MAO), which resulted in increased surface roughness, enhanced surface wettability and favourable mechanical properties. As compared with Ti-MAO, the TNZS-MAO samples with lower elastic modulus displayed increased cell attachment, alkaline phosphatase activity, collagen secretion, osteogenic marker expression, and mineralization of rat bone marrow mesenchymal stem cells. Upon implantation in rat femoral condylar defects, an inherently low elastic modulus could cooperatively accelerate early osseointegration and maturation of trabecular bone after 4 weeks implantation with the MAO modified surface. These results demonstrated that elastic modulus and the surface micro-scale topographical structure of Ti alloy implants have a synergistic effect on their osseointegration.
引用
收藏
页码:43685 / 43696
页数:12
相关论文
共 54 条
[1]
Early bone formation adjacent to rough and turned endosseous implant surfaces - An experimental study in the dog [J].
Abrahamsson, I ;
Berglundh, T ;
Linder, E ;
Lang, NP ;
Lindhe, J .
CLINICAL ORAL IMPLANTS RESEARCH, 2004, 15 (04) :381-392
[2]
Biomaterial strategies for engineering implants for enhanced osseointegration and bone repair [J].
Agarwal, Rachit ;
Garcia, Andres J. .
ADVANCED DRUG DELIVERY REVIEWS, 2015, 94 :53-62
[3]
Osteoinduction, osteoconduction and osseointegration [J].
Albrektsson, T ;
Johansson, C .
EUROPEAN SPINE JOURNAL, 2001, 10 (Suppl 2) :S96-S101
[4]
Enhanced bone apposition to a chemically modified SLA titanium surface [J].
Buser, D ;
Broggini, N ;
Wieland, M ;
Schenk, RK ;
Denzer, AJ ;
Cochran, DL ;
Hoffmann, B ;
Lussi, A ;
Steinemann, SG .
JOURNAL OF DENTAL RESEARCH, 2004, 83 (07) :529-533
[5]
Molecular mechanism of vinculin activation and nanoscale spatial organization in focal adhesions [J].
Case, Lindsay B. ;
Baird, Michelle A. ;
Shtengel, Gleb ;
Campbell, Sharon L. ;
Hess, Harald F. ;
Davidson, Michael W. ;
Waterman, Clare M. .
NATURE CELL BIOLOGY, 2015, 17 (07) :880-892
[6]
Calcium Plasma Implanted Titanium Surface with Hierarchical Microstructure for Improving the Bone Formation [J].
Cheng, Mengqi ;
Qiao, Yuqin ;
Wang, Qi ;
Jin, Guodong ;
Qin, Hui ;
Zhao, Yaochao ;
Peng, Xiaochun ;
Zhang, Xianlong ;
Liu, Xuanyong .
ACS APPLIED MATERIALS & INTERFACES, 2015, 7 (23) :13053-13061
[7]
Effect of surface roughness of hydroxyapatite on human bone marrow cell adhesion, proliferation, differentiation and detachment strength [J].
Deligianni, DD ;
Katsala, ND ;
Koutsoukos, PG ;
Missirlis, YF .
BIOMATERIALS, 2001, 22 (01) :87-96
[8]
Matrix elasticity directs stem cell lineage specification [J].
Engler, Adam J. ;
Sen, Shamik ;
Sweeney, H. Lee ;
Discher, Dennis E. .
CELL, 2006, 126 (04) :677-689
[9]
In Vivo Osseointegration of Nano-Designed Composite Coatings on Titanium Implants [J].
Facca, Sybille ;
Lahiri, Debrupa ;
Fioretti, Florence ;
Messadeq, Nadia ;
Mainard, Didier ;
Benkirane-Jessel, Nadia ;
Agarwal, Arvind .
ACS NANO, 2011, 5 (06) :4790-4799
[10]
Crosstalk between focal adhesions and material mechanical properties governs cell mechanics and functions [J].
Fusco, Sabato ;
Panzetta, Valeria ;
Embrione, Valerio ;
Netti, Paolo A. .
ACTA BIOMATERIALIA, 2015, 23 :63-71