Surface Plasmon Resonance Biosensor for Rapid Label-Free Detection of Microribonucleic Acid at Subfemtomole Level

被引:166
作者
Sipova, Hana [1 ]
Zhang, Shile [2 ]
Dudley, Aimee M. [2 ]
Galas, David [2 ]
Wang, Kai [2 ]
Homola, Jiri [1 ]
机构
[1] Acad Sci Czech Republ, Inst Photon & Elect, CR-18251 Prague, Czech Republic
[2] Inst Syst Biol, Seattle, WA 98103 USA
关键词
MICRORNA EXPRESSION; SENSOR;
D O I
10.1021/ac102131s
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Microribonucleic acids (miRNAs) have been linked with various regulatory functions and disorders, such as cancers and heart diseases. They, therefore, present an important target for detection technologies for future medical diagnostics. We report here a novel method for rapid and sensitive miRNA detection and quantitation using surface plasmon resonance (SPR) sensor technology and a DNA(star)RNA antibody-based assay. The approach takes advantage of a novel high-performance portable SPR sensor instrument for spectroscopy of surface plasmons based on a special diffraction grating called a surface plasmon coupler and disperser (SPRCD). The surface of the grating is functionalized with thiolated DNA oligonucleotides which specifically capture miRNA from a liquid sample without amplification. Subsequently, an antibody that recognizes DNA(star)RNA hybrids is introduced to bind to the DNA(star)RNA complex and enhance sensor response to the captured miRNA. This approach allows detection of miRNA in less than 30 min at concentrations down to 2 pM with an absolute amount at high attomoles. The methodology is evaluated for analysis of miRNA from mouse liver tissues and is found to yield results which agree well with those provided by the quantitative polymerase chain reaction (qPCR).
引用
收藏
页码:10110 / 10115
页数:6
相关论文
共 28 条
[1]   Expression profiling of microRNA using real-time quantitative PCR, how to use it and what is available [J].
Benes, Vladimir ;
Castoldi, Mirco .
METHODS, 2010, 50 (04) :244-249
[2]   Recent advances in DNA sensors [J].
Cosnier, Serge ;
Mailley, Pascal .
ANALYST, 2008, 133 (08) :984-991
[3]   Attomole microarray detection of MicroRNAs by nanoparticle-amplified SPR imaging measurements of surface polyadenylation reactions [J].
Fang, Shiping ;
Lee, Hye Jin ;
Wark, Alastair W. ;
Corn, Robert M. .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (43) :14044-14046
[4]   Direct multiplexed measurement of gene expression with color-coded probe pairs [J].
Geiss, Gary K. ;
Bumgarner, Roger E. ;
Birditt, Brian ;
Dahl, Timothy ;
Dowidar, Naeem ;
Dunaway, Dwayne L. ;
Fell, H. Perry ;
Ferree, Sean ;
George, Renee D. ;
Grogan, Tammy ;
James, Jeffrey J. ;
Maysuria, Malini ;
Mitton, Jeffrey D. ;
Oliveri, Paola ;
Osborn, Jennifer L. ;
Peng, Tao ;
Ratcliffe, Amber L. ;
Webster, Philippa J. ;
Davidson, Eric H. ;
Hood, Leroy .
NATURE BIOTECHNOLOGY, 2008, 26 (03) :317-325
[5]   Micrornas: Small RNAs with a big role in gene regulation [J].
He, L ;
Hannon, GJ .
NATURE REVIEWS GENETICS, 2004, 5 (07) :522-531
[6]   Colloidal Au-enhanced surface plasmon resonance for ultrasensitive detection of DNA hybridization [J].
He, L ;
Musick, MD ;
Nicewarner, SR ;
Salinas, FG ;
Benkovic, SJ ;
Natan, MJ ;
Keating, CD .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (38) :9071-9077
[7]   Surface plasmon resonance sensors for detection of chemical and biological species [J].
Homola, Jiri .
CHEMICAL REVIEWS, 2008, 108 (02) :462-493
[8]  
Hu Z. L., 2006, NUCLEIC ACIDS RES, P34
[9]   Altered microRNA expression in human heart disease [J].
Ikeda, Sadakatsu ;
Kong, Sek Won ;
Lu, Jun ;
Bisping, Egbert ;
Zhang, Hao ;
Allen, Paul D. ;
Golub, Todd R. ;
Pieske, Burkert ;
Pu, William T. .
PHYSIOLOGICAL GENOMICS, 2007, 31 (03) :367-373
[10]   MicroRNA gene expression deregulation in human breast cancer [J].
Iorio, MV ;
Ferracin, M ;
Liu, CG ;
Veronese, A ;
Spizzo, R ;
Sabbioni, S ;
Magri, E ;
Pedriali, M ;
Fabbri, M ;
Campiglio, M ;
Ménard, S ;
Palazzo, JP ;
Rosenberg, A ;
Musiani, P ;
Volinia, S ;
Nenci, I ;
Calin, GA ;
Querzoli, P ;
Negrini, M ;
Croce, CM .
CANCER RESEARCH, 2005, 65 (16) :7065-7070