Evaluation of methods used to determine ochratoxin a in coffee beans

被引:55
作者
Ahmed, N. E.
Farag, M. M.
Soliman, K. M.
Abdel-Samed, A. K. M.
Naguib, Kh. M. [1 ]
机构
[1] Natl Res Ctr, Dept Food Toxicol & Contaminants, Cairo, Egypt
[2] Cairo Univ, Fac Agr, Dept Biochem, Giza, Egypt
关键词
methods; ochratoxin A; coffee (green and roasted);
D O I
10.1021/jf072558x
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
A comparative study was conducted to evaluate four previously reported methods that proved to have a recovery greater than 80% for the determination of different levels of ochratoxin A (OTA) in green and roasted coffee beans and to select an accurate, sensitive, and less-expensive technique between the existing methods. The results indicated that the Association of Official Analytical Chemists (AOAC) official method for the extraction of OTA in green coffee and determination by high-performance liquid chromatography (HPLC) is recommended as an efficient method for the routine analyses of OTA in green and ground roasted coffee beans. This method proved to be an accurate, sensitive, and less-expensive method that employs routine materials and available equipment. Although the immunoaffinity column/HPLC procedure tested showed a significantly higher percentage than the AOAC recommended method, it is recommended for use in processed coffee beans where low concentrations of OTA may be expected to be detected.
引用
收藏
页码:9576 / 9580
页数:5
相关论文
共 28 条
[1]  
*ASS OFF AN CHEM, 2000, AOAC OFF METH AN ASS, V2
[2]  
*ASS OFF AN CHEM, 2000, OFF METH AN ASS OFF, V2
[3]  
BAUMANN U, 1988, MITT GEB LEB HUG, V79, P154
[4]  
DIRHEIMER G, 1991, IARC SCI PUBL, V115, P171
[5]  
ENGESTRON GW, 1977, J AGR FOOD CHEM, V25, P833
[6]  
HORWITZ W, 1993, J AOAC INT, V76, P461
[7]  
HULT K, 1976, J ASSOC OFF ANA CHEM, V59, P128
[8]   USE OF HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY COMBINED WITH FLUORESCENCE DETECTION FOR IDENTIFICATION AND ESTIMATION OF AFLATOXINS AND OCHRATOXIN IN FOOD [J].
HUNT, DC ;
BOURDON, AT ;
WILD, PJ ;
CROSBY, NT .
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 1978, 29 (03) :234-238
[9]   DETERMINATION OF OCHRATOXIN-A IN PIGS KIDNEY USING ENZYMIC DIGESTION, DIALYSIS AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH POST-COLUMN DERIVATIZATION [J].
HUNT, DC ;
PHILP, LA ;
CROSBY, NT .
ANALYST, 1979, 104 (1245) :1171-1175
[10]   HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF OCHRATOXIN-A IN ARTIFICIALLY SPIKED COCOA BEANS [J].
HURST, WJ ;
MARTIN, RA .
JOURNAL OF CHROMATOGRAPHY, 1983, 265 (02) :353-356