Structure of the lipopolysaccharide of Pseudomonas aeruginosa O-12 with a randomly O-acetylated core region

被引：26

作者：

Bystrova, OV

Lindner, B

Moll, H

Kocharova, NA

Knirel, YA

Zähringer, U

Pier, GB

机构：

[1] Russian Acad Sci, ND Zelinskii Organ Chem Inst, Moscow 119991, Russia

[2] Res Ctr Borstek, Ctr Med & Biosci, D-23845 Borstel, Germany

[3] Harvard Univ, Sch Med, Brigham & Womens Hosp, Channing Lab, Boston, MA 02115 USA

来源：

CARBOHYDRATE RESEARCH | 2003年 / 338卷 / 18期

关键词：

lipopolysaccharide; core structures; O-antigen repeating unit; O-acetylation; Pseudomonas aeruginosa;

D O I：

10.1016/S0008-6215(03)00290-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The lipopolysaccharide of Pseudomonas aeruginosa O-12 was studied by strong alkaline and mild acid degradations and dephosphorylation followed by fractionation of the products by GPC and high-performance anion-exchange chromatography and analyses by ESI FT-MS and NMR spectroscopy. The structures of the lipopolysaccharide core and the O-polysaccharide repeating unit were elucidated and the site and the configuration of the linkage between the O-polysaccharide and the core established. The core was found to be randomly O-acetylated, most O-acetyl groups being located on the terminal rhamnose residue of the outer core region (C) 2003 Elsevier Ltd. All rights reserved.

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页码：1895 / 1905

页数：11

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