ZnO thin film transistor immunosensor with high sensitivity and selectivity

被引：65

作者：

Reyes, Pavel Ivanoff ^{[1
]}

Ku, Chieh-Jen ^{[1
]}

Duan, Ziqing ^{[1
]}

Lu, Yicheng ^{[1
]}

Solanki, Aniruddh ^{[2
]}

Lee, Ki-Bum ^{[2
]}

机构：

[1] Rutgers State Univ, Dept Elect & Comp Engn, Piscataway, NJ 08854 USA

[2] Rutgers State Univ, Dept Chem & Chem Biol, Piscataway, NJ 08854 USA

来源：

APPLIED PHYSICS LETTERS | 2011年 / 98卷 / 17期

基金：

美国国家科学基金会;

关键词：

DEVICE;

D O I：

10.1063/1.3582555

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

A zinc oxide thin film transistor-based immunosensor (ZnO-bioTFT) is presented. The back-gate TFT has an on-off ratio of 108 and a threshold voltage of 4.25 V. The ZnO channel surface is biofunctionalized with primary monoclonal antibodies that selectively bind with epidermal growth factor receptor (EGFR). Detection of the antibody-antigen reaction is achieved through channel carrier modulation via pseudo double-gating field effect caused by the biochemical reaction. The sensitivity of 10 fM detection of pure EGFR proteins is achieved. The ZnO-bioTFT immunosensor also enables selectively detecting 10 fM of EGFR in a 5 mg/ml goat serum solution containing various other proteins. (C) 2011 American Institute of Physics. [doi:10.1063/1.3582555]

引用

页数：3

共 16 条

[1] Zinc oxide nanorod for intracellular pH sensing [J].

Al-Hilli, S. M. ;

Al-Mofarji, R. T. ;

Willander, M. .

APPLIED PHYSICS LETTERS, 2006, 89 (17)

[2]

Asahi M., 1975, JPN SOC APPL PHYS S, V44, P339

[3] Thirty years of ISFETOLOGY - What happened in the past 30 years and what may happen in the next 30 years [J].

Bergveld, P .

SENSORS AND ACTUATORS B-CHEMICAL, 2003, 88 (01) :1-20

[4] Detection of an uncharged steroid with a silicon nanowire field-effect transistor [J].

Chang, Ko Shing ;

Chen, Chen Chia ;

Sheu, Jeng Tzong ;

Li, Yaw-Kuan .

SENSORS AND ACTUATORS B-CHEMICAL, 2009, 138 (01) :148-153

[5] TiO2 nanowire FET device: Encapsulation of biomolecules by electro polymerized pyrrole propylic acid [J].

Chu, Yung-Ming ;

Lin, Chi-Chang ;

Chang, Hsien-Chang ;

Li, Changming ;

Guo, Chunxian .

BIOSENSORS & BIOELECTRONICS, 2011, 26 (05) :2334-2340

[6] Field effect detection of biomolecular interactions [J].

Estrela, P ;

Stewart, AG ;

Yan, F ;

Migliorato, P .

ELECTROCHIMICA ACTA, 2005, 50 (25-26) :4995-5000

[7] MICROSTRUCTURED SOLID-STATE ION-SENSITIVE MEMBRANES BY THERMAL-OXIDATION OF TA [J].

GIMMEL, P ;

SCHIERBAUM, KD ;

GOPEL, W ;

VANDENVLEKKERT, HH ;

DEROOIJ, NF .

SENSORS AND ACTUATORS B-CHEMICAL, 1990, 1 (1-6) :345-349

[8] Glucose sensor based on organic thin film transistor using glucose oxidase and conducting polymer [J].

Liu, J. ;

Agarwal, M. ;

Varahramyan, K. .

SENSORS AND ACTUATORS B-CHEMICAL, 2008, 135 (01) :195-199

[9] Chemical and biological sensors based on organic thin-film transistors [J].

Mabeck, JT ;

Malliaras, GG .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2006, 384 (02) :343-353

[10] Structural analysis of p185(c-neu) and epidermal growth factor receptor tyrosine kinases: Oligomerization of kinase domains [J].

Murali, R ;

Brennan, PJ ;

KieberEmmons, T ;

Greene, MI .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (13) :6252-6257

← 1 2 →