Propidium monoazide combined with real-time quantitative PCR underestimates heat-killed Listeria innocua

被引：96

作者：

Lovdal, Trond ^{[1
]}

Hovda, Maria Befring ^{[1
]}

Bjorkblom, Benny ^{[2
,3
]}

Moller, Simon G. ^{[2
,3
]}

机构：

[1] Nofima Norconserv AS, N-4068 Stavanger, Norway

[2] Univ Stavanger, Fac Sci & Technol, Ctr Organelle Res, N-4036 Stavanger, Norway

[3] Stavanger Univ Hosp, Norwegian Ctr Movement Disorders, N-4068 Stavanger, Norway

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2011年 / 85卷 / 02期

关键词：

Listeria innocua; Propidium monoazide; Quantitative real-time PCR; Viability; ETHIDIUM-BROMIDE MONOAZIDE; DEAD CELLS; MONOCYTOGENES CELLS; LIVE BACTERIA; ENUMERATION; REMOVAL; SAMPLES; EMA; DNA;

D O I：

10.1016/j.mimet.2011.01.027

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The combination of propidium monoazide (PMA) and quantitative real-time PCR (qPCR) significantly overestimated the fraction of viable Listeria innocua as compared to plate counts and confocal fluorescence microscopy. Our data imply that PMA-qPCR must be used with caution as an analytical tool for the differentiation between viable and dead bacteria. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：164 / 169

页数：6

共 16 条

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