Pathological features of transplanted tumor established by CD133 positive TJ905 glioblastoma stem-like cells

被引：14

作者：

Jin, Feng ^{[1
,2
]}

Zhang, Ran ^{[1
,2
]}

Feng, Song ^{[1
,2
]}

Yuan, Chuan-Tao ^{[3
]}

Zhang, Ren-Ya ^{[3
]}

Han, Guang-Kui ^{[1
,2
]}

Li, Gen-Hua ^{[1
,2
]}

Yu, Xi-Zhen ^{[1
,2
]}

Liu, Yang ^{[1
,2
]}

Kong, Ling-Sheng ^{[1
,2
]}

Zhang, Shu-Ling ^{[4
]}

Zhao, Lei ^{[4
]}

机构：

[1] Jining Med Univ, Affiliated Hosp, Dept Neurosurg, Jining 272029, Shandong, Peoples R China

[2] Shangdong Prov Key Lab Stem Cells & Neurooncol, Jining 272029, Shandong, Peoples R China

[3] Jining Med Univ, Affiliated Hosp, Dept Pathol, Jining 272029, Shandong, Peoples R China

[4] Huazhong Univ Sci & Technol, Tongji Med Coll, Union Hosp, Dept Infect Dis, Wuhan 430022, Peoples R China

来源：

CANCER CELL INTERNATIONAL | 2015年 / 15卷

基金：

美国国家科学基金会;

关键词：

CD133; Glioblastoma; Cancer stem-like cell; Animal model; Transplanted tumor; EXPRESSION; GROWTH; GENES;

D O I：

10.1186/s12935-015-0208-y

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

Background: This study is to explore the pathological features of transplanted tumor established by CD133 positive TJ905 glioblastoma stem-like cells. Methods: CD133 positive TJ905 glioma cells were separated by immunomagnetic beads to isolate glioma stem-like cells. TJ905 cells and stem-like cells were inoculated subcutaneously into the mice to establish model of transplanted tumor, respectively. Mice growing condition and behavior were observed. HE staining assay, immunohistochemical assay for GFAP, Ki-67 and Olig-2, and CD34 marked microvascular density (MVD) test were performed. Results: The growing condition and behavior of mice in TJ905 stem cell group was more exaggerated and the models showed stronger malignant features pathologically than that in TJ905 cell group. Glial fibrillary acidic protein (GFAP) in TJ905 cell and stem-like cell group showed the transplanted tumor originated from astrocytes. Expression of Ki-67 and oligodendrocyte transcription factor-2 (Olig-2) in TJ905 stem cells was higher notably and CD34 expression in stem cell group was significantly higher than that in the other two groups. Conclusions: Pathological features of transplanted tumor established by CD133 positive glioblastoma stem-like cells show more malignant. Use of TJ905 stem cells to establish transplanted tumor model in nude mice is excellent for glioma research.

引用

页数：9

共 22 条

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