Diffractive micro bar codes for encoding of biomolecules in multiplexed assays

被引：29

作者：

Broder, Graham R. ^{[1
]}

Ranasinghe, Rohan T. ^{[1
]}

She, Joseph K. ^{[1
]}

Banu, Shahanara ^{[1
,2
]}

Birtwell, Sam W. ^{[3
,4
]}

Cavalli, Gabriel ^{[1
]}

Galitonov, Gerasim S. ^{[3
]}

Holmes, David ^{[2
]}

Martins, Hugo F. P. ^{[1
]}

MacDonald, Kevin F. ^{[3
]}

Neylon, Cameron ^{[1
,5
]}

Zheludev, Nikolay ^{[3
]}

Roach, Peter L. ^{[1
]}

Morgan, Hywel ^{[2
]}

机构：

[1] Univ Southampton, Sch Chem, Southampton SO17 1BJ, Hants, England

[2] Univ Southampton, Sch Elect & Comp Sci, Southampton SO17 1BJ, Hants, England

[3] Univ Southampton, Optoelect Res Ctr, Southampton SO17 1BJ, Hants, England

[4] Univ Southampton, Sch Phys & Astron, Southampton SO17 1BJ, Hants, England

[5] Rutherford Appleton Lab, STFC, Didcot OX11 0QX, Oxon, England

来源：

ANALYTICAL CHEMISTRY | 2008年 / 80卷 / 06期

基金：

英国工程与自然科学研究理事会;

关键词：

D O I：

10.1021/ac7018574

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Microparticles incorporating micrometer-sized diffractive bar codes have been modified with oligonucleotides and immunoglobulin Gs to enable DNA hybridization and immunoassays. The bar codes are manufactured using photolithography of a chemically functional commercial epoxy photoresist (SU-8). When attached by suitable linkers, immobilized probe molecules exhibit high affinity for analytes and fast reaction kinetics, allowing detection of single nucleotide differences in DNA sequences and multiplexed immunoassays in <45 min. Analysis of raw data from assays carried out on the diffractive microparticles indicates that the reproducibility and sensitivity approach those of commercial encoding platforms. Micrometer-sized particles, imprinted with several super-imposed diffraction gratings, can encode many million unique codes. The high encoding capacity of this technology along with the applicability of the manufactured bar codes to multiplexed assays will allow accurate measurement of a wide variety of molecular interactions, leading to new opportunities in diverse areas of biotechnology such as genomics, proteomics, high-throughput screening, and medical diagnostics.

引用

页码：1902 / 1909

页数：8

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