Multicolor chromosome painting in diagnostic and research applications

被引:29
作者
Langer, S
Kraus, J
Jentsch, I
Speicher, MR
机构
[1] Tech Univ Munich, Inst Humangenet, D-81675 Munich, Germany
[2] GSF Forschungszentrum Umwelt & Gesundheit GMBH, Inst Humangenet, D-85764 Neuherberg, Germany
关键词
centromere; chromosome painting; clinical cytogenetics; DNA probes; in situ hybridization; telomere;
D O I
10.1023/B:CHRO.0000009326.21752.88
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
For many years whole chromosome painting probes have been the work-horses in a large variety of clinical and research molecular cytogenetic applications. In recent years painting probes have been complemented by an increasing number of further region-specific probes, which allow the specific staining of centromeres, subtelomeres or other regions within the genome. This development of new probe sets was greatly facilitated by the Human Genome Project from which well-characterized probes for any region within the genome have emerged. Furthermore, the evolution of different multicolor fluorescence in situ hybridization ( FISH) technologies now allows the cohybridization of multiple DNA-probes of different colors. These developments have paved the way for FISH-based automated karyotyping or the simultaneous analysis of multiple defined regions within the genome. Using appropriate instrumentation and image processing, the analysis can be performed two-dimensionally on metaphase spreads or three-dimensionally in intact interphase nuclei. Here we summarize some of the most recent developments and discuss the application of painting probes in different scenarios.
引用
收藏
页码:15 / 23
页数:9
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