Effect of gamma-ray on activity and stability of alcohol-dehydrogenase from Saccharomyces cerevisiae

被引：14

作者：

Ben Akacha, Najla ^{[1
]}

Zehlila, Amel ^{[1
]}

Merji, Sonia ^{[2
]}

Jerbi, Taieb ^{[2
]}

Gargouri, Mohamed ^{[1
]}

机构：

[1] Natl Inst Appl Sci & Technol INSAT, Biocatalysis & Bioproc Unit, Enzyme Engn Grp, Tunis, Tunisia

[2] Natl Ctr Nucl Sci & Technol CNSTN, Tunis, Tunisia

来源：

BIOCHEMICAL ENGINEERING JOURNAL | 2008年 / 40卷 / 01期

关键词：

alcohol-dehydrogenase; entrapment; immobilization; gamma-ray; Saccharomyces cerevisiae;

D O I：

10.1016/j.bej.2007.12.006

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The effect of gamma-ray irradiation on alcohol-dehydrogenase activity of yeast was investigated. The results suggested that low doses of gamma-ray (10 and 20 Gy) significantly increased the enzyme activity. This work also describes the impact of irradiation on immobilization efficiency of biocatalyst entrapped on to alginate gel beads. When yeast it-radiated to a dose of 20Gy was immobilized, ADH stability was improved up to 1.4 times at 45 degrees C compared to the immobilized non-irradiated cells. Also, the irradiated biocatalyst, when immobilized, can be reused more than eight times in oxidation reaction of ethanol. This preparation also permitted to reach high yields of immobilization (79%) and activity (88%). (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：184 / 188

页数：5

共 18 条

[1] Co-immobilization of cyclohexanone monooxygenase and glucose-6-phosphate dehydrogenase onto polyethylenimine-porous agarose polymeric composite using γ irradiation to use in biotechnological processes [J].

Atia, KS .

RADIATION PHYSICS AND CHEMISTRY, 2005, 73 (02) :91-99

[2] Production of hexenol in a two-enzyme system: kinetic study and modelling [J].

Akacha, NB ;

Boubaker, O ;

Gargouri, M .

BIOTECHNOLOGY LETTERS, 2005, 27 (23-24) :1875-1878

[3] Enhancement of regeneration potential and variability by γ-irradiation in cultured cells of Scilla indica [J].

Chakravarty, B ;

Sen, S .

BIOLOGIA PLANTARUM, 2001, 44 (02) :189-193

[4] CAN IMMOBILIZATION BE EXPLOITED TO MODIFY ENZYME-ACTIVITY [J].

CLARK, DS .

TRENDS IN BIOTECHNOLOGY, 1994, 12 (11) :439-443

[5] Stabilization of α-amylase by using anionic surfactant during the immobilization process [J].

El-Batal, AI ;

Atia, KS ;

Eid, M .

RADIATION PHYSICS AND CHEMISTRY, 2005, 74 (02) :96-101

[6] Coupled hydroperoxide lyase and alcohol dehydrogenase for selective synthesis of aldehyde or alcohol [J].

Gargouri, M ;

Ben Akacha, N ;

Legoy, MD .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2004, 119 (02) :171-180

[7] IMMOBILIZATION OF SACCHAROMYCES-CEREVISIAE FOR ETHANOL FERMENTATION ON GAMMA-ALUMINA PARTICLES USING A SPRAY-DRYER [J].

ISONO, Y ;

ARAYA, G ;

HOSHINO, A .

PROCESS BIOCHEMISTRY, 1995, 30 (08) :743-746

[8] DAMAGE TO PROTEINS DUE TO THE DIRECT ACTION OF IONIZING-RADIATION [J].

KEMPNER, ES .

QUARTERLY REVIEWS OF BIOPHYSICS, 1993, 26 (01) :27-48

[9]

Mateo C, 2000, BIOTECHNOL BIOENG, V68, P98, DOI 10.1002/(SICI)1097-0290(20000405)68:1<98::AID-BIT12>3.0.CO

[10]

2-T

← 1 2 →