Analysis of Hepatitis C Virus Intrahost Diversity across the Coding Region by Ultradeep Pyrosequencing

被引：40

作者：

Lauck, Michael ^{[1
]}

Alvarado-Mora, Monica V. ^{[2
,3
]}

Becker, Ericka A. ^{[1
]}

Bhattacharya, Dipankar ^{[5
,6
]}

Striker, Rob ^{[4
,5
,6
]}

Hughes, Austin L. ^{[7
]}

Carrilho, Flair J. ^{[2
,3
]}

O'Connor, David H. ^{[1
]}

Rebello Pinho, Joao R. ^{[2
,3
]}

机构：

[1] Univ Wisconsin, Dept Pathol & Lab Med, Madison, WI 53703 USA

[2] Univ Sao Paulo, Sch Med, Sao Paulo Inst Trop Med, Lab Trop Gastroenterol & Hepatol, Sao Paulo, Brazil

[3] Univ Sao Paulo, Sch Med, Dept Gastroenterol, Sao Paulo, Brazil

[4] WS Middleton Mem Vet Assoc Hosp, Madison, WI USA

[5] Univ Wisconsin, Dept Med, Madison, WI USA

[6] Univ Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 USA

[7] Univ S Carolina, Dept Biol Sci, Columbia, SC 29208 USA

来源：

JOURNAL OF VIROLOGY | 2012年 / 86卷 / 07期

基金：

巴西圣保罗研究基金会; 美国国家卫生研究院;

关键词：

RESISTANCE MUTATIONS; SELECTION PRESSURE; PROTEASE; HCV; VARIANTS; REPLICATION; BOCEPREVIR; INHIBITORS; POPULATION; TELAPREVIR;

D O I：

10.1128/JVI.06627-11

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Hepatitis C virus (HCV) is the leading cause of liver disease worldwide. In this study, we analyzed four treatment-naive patients infected with subtype 1a and performed Roche/454 pyrosequencing across the coding region. We report the presence of low-level drug resistance mutations that would most likely have been missed using conventional sequencing methods. The approach described here is broadly applicable to studies of viral diversity and could help to improve the efficacy of direct-acting antiviral agents (DAA) in the treatment of HCV-infected patients.

引用

页码：3952 / 3960

页数：9

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