The role of DNA binding with the Cry8Ea1 toxin of Bacillus thuringiensis

被引：6

作者：

Guo, Shuyuan ^{[2
]}

Li, Jie ^{[2
]}

Liu, Yanfeng ^{[1
]}

Song, Fuping ^{[1
]}

Zhang, Jie ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

[2] Beijing Inst Technol, Sch Life Sci, Beijing 100081, Peoples R China

来源：

FEMS MICROBIOLOGY LETTERS | 2011年 / 317卷 / 02期

关键词：

Bacillus thuringiensis; delta-endotoxin; toxin-DNA; protoxin-DNA; aggregation; DELTA-ENDOTOXIN; CRYSTAL PROTEIN; INSERTION; MEMBRANES; GENE;

D O I：

10.1111/j.1574-6968.2011.02230.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Cry8Ea1 protoxin is a DNA-protein complex. Both forms of the Cry8Ea1 toxin (with or without DNA binding) were obtained separately, and their stability and ability to insert into a phospholipid monolayer in vitro were compared. The presence of DNA can prevent the toxin from aggregation. Data regarding the penetration of the Cry8Ea1 toxin and Cry8Ea1 toxin-DNA complex into the air/water interface without a phospholipid monolayer show that the Cry8Ea1 toxin-DNA complex is more likely to move towards the air/water interface and is more hydrophobic. Experiments examining the protein interaction with the phospholipid monolayer show that the ability of the Cry8Ea1 toxin-DNA complex to insert into the lipid bilayer is much greater than that of the Cry8Ea1 toxin without DNA.

引用

页码：203 / 210

页数：8

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