Tip-enhanced Raman spectroscopy of single RNA strands: Towards a novel direct-sequencing method

被引：266

作者：

Bailo, Elena ^{[1
]}

Deckert, Volker ^{[1
]}

机构：

[1] Inst Sci Anal, ISAS, D-44139 Dortmund, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2008年 / 47卷 / 09期

关键词：

Raman spectroscopy; RNA; scanning probe microscopy; sequencing; surface analysis;

D O I：

10.1002/anie.200704054

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Walking the strand: Tip-enhanced Raman scattering (TERS) with precision probe positioning has been used to obtain high-quality Raman spectra of the nucleobases in a single RNA strand (see picture: foreground Raman spectrum, background atomic-force microscope tip positioned over the RNA strand). This procedure represents the first step towards direct and label-free single-biomolecule sequencing. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

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页码：1658 / 1661

页数：4

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