KNOX homeobox genes are sufficient in maintaining cultured cells in an undifferentiated state in rice

被引:35
作者
Ito, Y [1 ]
Eiguchi, M [1 ]
Kurata, N [1 ]
机构
[1] Natl Inst Genet, Plant Genet Lab, Mishima, Shizuoka, Japan
关键词
shoot meristem; regeneration; development; OSH genes; transformation; Oryza sativa;
D O I
10.1002/gene.1069
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
We produced transgenic rice calli, which constitutively express each of four KNOX family class 1 homeobox genes of rice, OSH1, OSH16, OSH15, and OSH71, and found that constitutive and ectopic expression of such genes inhibits normal regeneration from transformed calli, which showed continuous growth around their shoot-regenerating stages. Transgenic call! transferred onto regeneration medium began to display green spots, a sign of regeneration, but most of the transformants continued to propagate green spots at given stages. In the normal shoot-regeneration process of calli, expression of endogenous OSH1 was restricted in presumptive shoot-regenerating regions of calli and not observed in other areas. This restricted expression pattern should be required for further differentiation of the regenerating shoots. Thus our present results support the proposed function that KNOX family class 1 homeobox genes play a role in the formation and maintenance of the undetermined meristematic state of cells. (C) 2001 Wiley-Liss, Inc.
引用
收藏
页码:231 / 238
页数:8
相关论文
共 27 条
[11]   A KNOTTED1-LIKE HOMEOBOX GENE IN ARABIDOPSIS IS EXPRESSED IN THE VEGETATIVE MERISTEM AND DRAMATICALLY ALTERS LEAF MORPHOLOGY WHEN OVEREXPRESSED IN TRANSGENIC PLANTS [J].
LINCOLN, C ;
LONG, J ;
YAMAGUCHI, J ;
SERIKAWA, K ;
HAKE, S .
PLANT CELL, 1994, 6 (12) :1859-1876
[12]   A member of the KNOTTED class of homeodomain proteins encoded by the STM gene of Arabidopsis [J].
Long, JA ;
Moan, EI ;
Medford, JI ;
Barton, MK .
NATURE, 1996, 379 (6560) :66-69
[13]   EXPRESSION OF A RICE HOMEOBOX GENE CAUSES ALTERED MORPHOLOGY OF TRANSGENIC PLANTS [J].
MATSUOKA, M ;
ICHIKAWA, H ;
SAITO, A ;
TADA, Y ;
FUJIMURA, T ;
KANOMURAKAMI, Y .
PLANT CELL, 1993, 5 (09) :1039-1048
[14]   Role of WUSCHEL in regulating stem cell fate in the Arabidopsis shoot meristem [J].
Mayer, KFX ;
Schoof, H ;
Haecker, A ;
Lenhard, M ;
Jürgens, G ;
Laux, T .
CELL, 1998, 95 (06) :805-815
[15]  
OHTA S, 1990, PLANT CELL PHYSIOL, V31, P805
[16]   Knots in the family tree:: evolutionary relationships and functions of knox homeobox genes [J].
Reiser, L ;
Sánchez-Baracaldo, P ;
Hake, S .
PLANT MOLECULAR BIOLOGY, 2000, 42 (01) :151-166
[17]   KNOX homeodomain protein directly suppresses the expression of a gibberellin biosynthetic gene in the tobacco shoot apical meristem [J].
Sakamoto, T ;
Kamiya, N ;
Ueguchi-Tanaka, M ;
Iwahori, S ;
Matsuoka, M .
GENES & DEVELOPMENT, 2001, 15 (05) :581-590
[18]  
Sato T, 1996, MOL GEN GENET, V251, P13
[19]   A rice homeobox gene, OSH1, is expressed before organ differentiation in a specific region during early embryogenesis [J].
Sato, Y ;
Hong, SK ;
Tagiri, A ;
Kitano, H ;
Yamamoto, N ;
Nagato, Y ;
Matsuoka, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (15) :8117-8122
[20]   Loss-of-function mutations in the rice homeobox gene OSH15 affect the architecture of internodes resulting in dwarf plants [J].
Sato, Y ;
Sentoku, N ;
Miura, Y ;
Hirochika, H ;
Kitano, H ;
Matsuoka, M .
EMBO JOURNAL, 1999, 18 (04) :992-1002