Single cell metabolomics

被引：89

作者：

Heinemann, Matthias ^{[1
,2
]}

Zenobi, Renato ^{[3
]}

机构：

[1] Swiss Fed Inst Technol, Inst Mol Syst Biol, CH-8093 Zurich, Switzerland

[2] Univ Groningen, Groningen Biomol Sci & Biotechnol Inst, NL-9747 AG Groningen, Netherlands

[3] Swiss Fed Inst Technol, Dept Chem & Appl Biosci, CH-8093 Zurich, Switzerland

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2011年 / 22卷 / 01期

关键词：

DESORPTION/IONIZATION MASS-SPECTROMETRY; STOCHASTIC GENE-EXPRESSION; CAPILLARY-ELECTROPHORESIS; MICROFLUIDIC DEVICE; ESCHERICHIA-COLI; METABOLITES; YEAST;

D O I：

10.1016/j.copbio.2010.09.008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent discoveries suggest that cells of a clonal population often display multiple metabolic phenotypes at the same time. Motivated by the success of mass spectrometry (MS) in the investigation of population-level metabolomics, the analytical community has initiated efforts towards MS-based single cell metabolomics to investigate metabolic phenomena that are buried under the population average. Here, we review the current approaches and illustrate their advantages and disadvantages. Because of significant advances in the field, different technologies are now at the verge of generating data that are useful for exploring and investigating metabolic heterogeneity.

引用

页码：26 / 31

页数：6

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