Application of a metabolomic method combining one-dimensional and two-dimensional gas chromatography-time-of-flight/mass spectrometry to metabolic phenotyping of natural variants in rice

被引：121

作者：

Kusano, Miyako ^{[1
]}

Fukushima, Atsushi

Kobayashi, Makoto

Hayashi, Naomi

Jonsson, Paer

Moritz, Thomas

Ebana, Kaworu

Saito, Kazuki

机构：

[1] RIKEN, Plant Sci Ctr, Yokohama, Kanagawa 2300045, Japan

[2] Umea Univ, Dept Chem, Grp Chemometr Organ Chem, SE-90187 Umea, Sweden

[3] Swedish Univ Agr Sci, Dept Forest Genet & Plant Physiol, Umea Plant Sci Ctr, SE-90187 Umea, Sweden

[4] Natl Inst Agrobiol Sci, Tsukuba, Ibaraki 3058602, Japan

[5] Chiba Univ, Grad Sch Pharmaceut Sci, Dept Mol Biol & Biotechnol, Chiba 2638522, Japan

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2007年 / 855卷 / 01期

关键词：

rice core collection; GC x GC; two-dimensional chromatography; gas chromatography; metabolic phenotyping;

D O I：

10.1016/j.jchromb.2007.05.002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a comprehensive method combining analytical techniques of one-dimensional (I D) and two-dimensional (GC x GC) gas chromatography-time-of-flight (TOF)-mass spectrometry. This method was applied to the metabolic phenotyping of natural variants in rice for the 68 world rice core collection (WRC) and two other varieties. Ten metabolites were selected as metabolite representatives, and the selected ion current of each metabolite peak obtained from both techniques were statistically compared. Our method of combining I D- and GC x GC-TOF/MS is useful for the metabolic phenotyping of natural variants in rice for further studies in breeding programs. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：71 / 79

页数：9

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