Transplantation of SNAP-treated adipose tissue-derived stem cells improves cardiac function and induces neovascularization after myocardium infarct in rats

被引：20

作者：

Berardi, Gel R. M. ^{[3
]}

Rebelatto, Carmen K. ^{[3
]}

Tavares, Heloisa F. ^{[3
]}

Ingberman, Max

Shigunov, Patricia ^{[2
]}

Barchiki, Fabiane ^{[3
]}

Aguiar, Alessandra M. ^{[2
]}

Miyague, Nelson I. ^{[3
]}

Francisco, Julio C. ^{[3
]}

Correa, Alejandro ^{[2
]}

Senegaglia, Alexandra C. ^{[3
]}

Suss, Paula Hansen ^{[3
]}

Moutinho, Jose A. ^{[3
]}

Sotomaior, Vanessa S. ^{[3
]}

Nakao, Lia S. ^{[1
,3
]}

Brofman, Paulo S. ^{[3
]}

机构：

[1] Univ Fed Parana, Ctr Politecn, Dept Patol Basica, Setor Ciencias Biol, BR-81531980 Curitiba, Parana, Brazil

[2] Fiocruz MS, Inst Carlos Chagas, BR-81350010 Curitiba, Parana, Brazil

[3] Pontificia Univ Catolica Parana, BR-80215901 Curitiba, Parana, Brazil

来源：

EXPERIMENTAL AND MOLECULAR PATHOLOGY | 2011年 / 90卷 / 02期

关键词：

Adipose-derived stem cells; SNAP; Nitric oxide; Ejection fraction; Neovascularization; Cardiomyogenesis; NITRIC-OXIDE; BONE-MARROW; STROMAL CELLS; COLLATERAL PERFUSION; OXIDATIVE STRESS; S-NITROSOTHIOLS; MODEL; REGENERATION; HEART; DIFFERENTIATION;

D O I：

10.1016/j.yexmp.2010.11.005

中图分类号：

R36 [病理学];

学科分类号：

100103 [病原生物学];

摘要：

Stem cell therapy has been considered a promise for damaged myocardial tissue. We have previously shown that S-nitroso-N-acetyl-D,L-penicillamine (SNAP) increases the expression of several muscular markers and VEGF in mesenchymal stem cells, indicating that transplantation of SNAP-treated cells could provide better functional outcomes. Here, we transplanted SNAP-treated adipose tissue-derived stem cells (ADSCs) in rat infarcted myocardium. After 30 days, we observed a significant improvement of the ejection fraction in rats that received SNAP-treated ADSCs, compared with those that received untreated cells (p = 0.008). Immunohistochemical reactions showed an increased expression of troponin T-C and von Willebrand factor, and organized vascular units in the infarcted area of tissue transplanted with treated ADSCs. SNAP exposure induced intracellular S-nitrosation, a decreased GSH/GSSG ratio, but did not increase cGMP levels. Collectively, these results indicate that SNAP alters the redox environment of ADSCs, possibly associated with a pre-differentiation state, which may improve cardiac function after transplantation. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：149 / 156

页数：8

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