Kanamycin is effective for selecting transformed peas

被引：24

作者：

Grant, JE ^{[1
]}

Cooper, PA ^{[1
]}

Gilpin, BJ ^{[1
]}

Hoglund, SJ ^{[1
]}

Reader, JK ^{[1
]}

Pither-Joyce, MD ^{[1
]}

Timmerman-Vaughan, GM ^{[1
]}

机构：

[1] New Zealand Inst Crop & Food Res Ltd, Christchurch 4704, New Zealand

来源：

PLANT SCIENCE | 1998年 / 139卷 / 02期

关键词：

transformation; kanamycin; peas; Pisum sativum;

D O I：

10.1016/S0168-9452(98)00184-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Immature cotyledons from six cultivars and breeding lines of Pisum sativum were cocultivated with Agrobacterium tumefaciens and selected on a medium containing kanamycin. Transformed plants were recovered with a success rate of between 0.8 and 3.4% of explants treated. The plasmids used were based on pGA633 and have a nos promoter driving an nptII gene. Progeny from the primary transformants showed stable inheritance of the nos-nptII gene. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：159 / 164

页数：6

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