Arabidopsis ROOT UVB SENSITIVE2/WEAK AUXIN RESPONSE1 Is Required for Polar Auxin Transport

被引：37

作者：

Ge, L. ^{[1
]}

Peer, W. ^{[2
]}

Robert, S. ^{[3
,4
]}

Swarup, R. ^{[5
,6
]}

Ye, S. ^{[7
,8
]}

Prigge, M. ^{[1
]}

Cohen, J. D. ^{[7
,8
]}

Friml, J. ^{[3
,4
]}

Murphy, A. ^{[2
]}

Tang, D. ^{[9
]}

Estelle, M. ^{[1
]}

机构：

[1] Univ Calif San Diego, La Jolla, CA 92093 USA

[2] Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47907 USA

[3] Univ Ghent, Dept Plant Syst Biol, Flanders Inst Biotechnol, B-9053 Ghent, Belgium

[4] Univ Ghent, Dept Plant Biotechnol & Genet, B-9053 Ghent, Belgium

[5] Univ Nottingham, Sch Biosci, Loughborough LE12 5RD, Leics, England

[6] Univ Nottingham, Ctr Plant Integrat Biol, Loughborough LE12 5RD, Leics, England

[7] Univ Minnesota, Dept Hort Sci, St Paul, MN 55108 USA

[8] Univ Minnesota, Microbial & Plant Genom Inst, St Paul, MN 55108 USA

[9] Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China

来源：

PLANT CELL | 2010年 / 22卷 / 06期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

REGULATES ROOT GRAVITROPISM; BOX PROTEIN TIR1; PLANT DEVELOPMENT; ARABIDOPSIS-THALIANA; EFFLUX CARRIER; PIN PHOSPHORYLATION; DOMINANT MUTATION; PERMEASE AUX1; TRAFFICKING; GROWTH;

D O I：

10.1105/tpc.110.074195

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Auxin is an essential phytohormone that regulates many aspects of plant development. To identify new genes that function in auxin signaling, we performed a genetic screen for Arabidopsis thaliana mutants with an alteration in the expression of the auxin-responsive reporter DR5rev:GFP (for green fluorescent protein). One of the mutants recovered in this screen, called weak auxin response1 (wxr1), has a defect in auxin response and exhibits a variety of auxin-related growth defects in the root. Polar auxin transport is reduced in wxr1 seedlings, resulting in auxin accumulation in the hypocotyl and cotyledons and a reduction in auxin levels in the root apex. In addition, the levels of the PIN auxin transport proteins are reduced in the wxr1 root. We also show that WXR1 is ROOT UV-B SENSITIVE2 (RUS2), a member of the broadly conserved DUF647 domain protein family found in diverse eukaryotic organisms. Our data indicate that RUS2/WXR1 is required for auxin transport and to maintain the normal levels of PIN proteins in the root.

引用

页码：1749 / 1761

页数：13

共 63 条

[1] Intracellular trafficking and proteolysis of the Arabidopsis auxin-efflux facilitator PIN2 are involved in root gravitropism [J].