Is meconium from healthy newborns actually sterile?

被引：689

作者：

Jimenez, Esther ^{[1
]}

Marin, Maria L. ^{[1
]}

Martin, Rocio ^{[1
]}

Odriozola, Juan M. ^{[2
]}

Olivares, Monica ^{[3
]}

Xaus, Jordi ^{[3
]}

Fernandez, Leonides ^{[1
]}

Rodriguez, Juan M. ^{[1
]}

机构：

[1] Univ Complutense, Dept Nutr Bromatol & Tecnol Alimentos, E-28040 Madrid, Spain

[2] Hosp Univ Doce Octubre, Serv Obstet, Madrid 28041, Spain

[3] Puleva Biotech, Dept Biomed, Granada 18004, Spain

来源：

RESEARCH IN MICROBIOLOGY | 2008年 / 159卷 / 03期

关键词：

meconium; gut; fetus; Enterococcus; Staphylococcus;

D O I：

10.1016/j.resmic.2007.12.007

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In a previous study, bacteria were able to be isolated from umbilical cord blood of healthy neonates and from murine amniotic fluid obtained by caesarean section. This suggested that term fetuses are not completely sterile and that a prenatal mother-to-child efflux of commensal bacteria may exist. Therefore, the presence of such bacteria in meconium of 21 healthy neonates was investigated. The identified isolates belonged predominantly to the genuses Enterococcus and Staphylococcus. Later, a group of pregnant mice were orally inoculated with a genetically labelled E. fecium strain previously isolated from breast milk of a healthy woman. The labelled strain could be isolated and PCR-detected from meconium of the inoculated animals obtained by caesarean section one day before the predicted date of labor. In contrast, it could not be detected in samples obtained from a non-inoculated control group. (C) 2008 Elsevier Masson SAS. All rights reserved.

引用

页码：187 / 193

页数：7

共 38 条

[1] Reduced enterobacterial and increased staphylococcal colonization of the infantile bowel:: An effect of hygienic lifestyle? [J].

Adlerberth, I ;

Lindberg, E ;

Åberg, N ;

Hesselmar, B ;

Saalman, R ;

Strannegård, IL ;

Wold, AE .

PEDIATRIC RESEARCH, 2006, 59 (01) :96-101

[2] DIET AND FECAL FLORA IN THE NEWBORN - BREAST-MILK AND INFANT FORMULA [J].

BALMER, SE ;

WHARTON, BA .

ARCHIVES OF DISEASE IN CHILDHOOD, 1989, 64 (12) :1672-1677

[3] Possible association between amniotic fluid micro-organism infection and microflora in the mouth [J].

Bearfield, C ;

Davenport, ES ;

Sivapathasundaram, V ;

Allaker, RP .

BJOG-AN INTERNATIONAL JOURNAL OF OBSTETRICS AND GYNAECOLOGY, 2002, 109 (05) :527-533

[4] DEVELOPMENT OF AN ELECTROPORATION PROCEDURE FOR GENE DISRUPTION IN LACTOBACILLUS-HELVETICUS CNRZ-32 [J].

BHOWMIK, T ;

STEELE, JL .

JOURNAL OF GENERAL MICROBIOLOGY, 1993, 139 :1433-1439

[5]

Borderon JC, 1996, PATHOL BIOL, V44, P416

[6] Specific proliferative and antibody responses of premature infants to intestinal colonization with nonpathogenic probiotic E-coli strain Nissle 1917 [J].

Cukrowska, B ;

Lodínova-Zádníková, R ;

Enders, C ;

Sonnenborn, U ;

Schulze, J ;

Tlaskalová-Hogenová, H .

SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 2002, 55 (02) :204-209

[7] Salivary Actinomyces naeslundii genospecies 2 and Lactobacillus casei levels predict pregnancy outcomes [J].

Dasanayake, AP ;

Li, YH ;

Wiener, H ;

Ruby, JD ;

Lee, MJ .

JOURNAL OF PERIODONTOLOGY, 2005, 76 (02) :171-177

[8] A CASSETTE VECTOR FOR PROTEIN ENGINEERING THE LANTIBIOTIC NISIN [J].

DODD, HM ;

HORN, N ;

GASSON, MJ .

GENE, 1995, 162 (01) :163-164

[9] The effect of meconium staining of amniotic fluid on the growth of Escherichia coli and group B Streptococcus [J].

Eidelman A.I. ;

Nevet A. ;

Rudensky B. ;

Rabinowitz R. ;

Hammerman C. ;

Raveh D. ;

Schimmel M.S. .

Journal of Perinatology, 2002, 22 (6) :467-471

[10] Molecular monitoring of succession of bacterial communities in human neonates [J].

Favier, CF ;

Vaughan, EE ;

De Vos, WM ;

Akkermans, ADL .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (01) :219-226

← 1 2 3 4 →