Design of biotin-functionalized luminescent quantum dots

被引：17

作者：

Susumu, Kimihiro ^{[1
]}

Uyeda, H. Tetsuo ^{[1
,2
]}

Medintz, Igor L. ^{[3
]}

Mattoussi, Hedi ^{[1
]}

机构：

[1] USN, Res Lab, Div Opt Sci, Washington, DC 20375 USA

[2] Promega Biosci Inc, San Luis Obispo, CA 93401 USA

[3] USN, Res Lab, Ctr Biomol Sci & Engn, Washington, DC 20375 USA

来源：

JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY | 2007年

关键词：

D O I：

10.1155/2007/90651

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We report the design and synthesis of a tetraethylene glycol-(TEG-) based bidentate ligand functionalized with dihydrolipoic acid (DHLA) and biotin (DHLA-TEG-biotin) to promote biocompatibility of luminescent quantum dots (QD's). This new ligand readily binds to CdSe-ZnS core-shell QDs via surface ligand exchange. QDs capped with a mixture of DHLA and DHLA-TEG biotin or polyethylene glycol-(PEG-) (molecular weight average similar to 600) modified DHLA (DHLA-PEG600) and DHLA-TEG biotin are easily dispersed in aqueous bu. er solutions. In particular, homogeneous bu. er solutions of QDs capped with a mixture of DHLA-PEG600 and DHLA-TEG-biotin that are stable over broad pH range have been prepared. QDs coated with mixtures of DHLA/DHLA-TEG-biotin and with DHLA-PEG600/DHLA-TEG-biotin were tested in surface binding assays and the results indicate that biotin groups on the QD surface interact specifically with NeutrAvidin-functionalized microtiter well plates. Copyright (C) 2007 Kimihiro Susumu et al.

引用

页数：7

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