Increase of nitric oxide synthases and nitrotyrosine in inclusion-body myositis

被引：65

作者：

Yang, CC ^{[1
]}

Alvarez, RB ^{[1
]}

Engel, WK ^{[1
]}

Askanas, V ^{[1
]}

机构：

[1] UNIV SO CALIF,SCH MED,GOOD SAMARITAN HOSP,NEUROMUSCULAR CTR,DEPT NEUROL,LOS ANGELES,CA 90017

来源：

NEUROREPORT | 1996年 / 8卷 / 01期

关键词：

immunocytochemistry; inclusion-body myositis; nitric oxide; nitric oxide synthase; nitrotyrosine;

D O I：

10.1097/00001756-199612200-00031

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

To investigate the possible role of nitric oxide (NO)induced 'oxidative stress' in the pathogenesis of inclusion-body myositis (IBM), we immunostained muscle biopsies of 12 patients with IBM with isoform-specific antibodies against the neuronal and inducible forms of nitric oxide synthase and with antibodies against nitrotyrosine. Between 70 and 80% of IBM vacuolated muscle fibers contained inclusions strongly immunoreactive with all three antibodies, which by immuno-electronmicroscopy co-localized mainly to cytoplasmic paired-helical filaments, and also to amorphous structures and floccular material. Excess intracellular NO can combine with superoxide to produce highly reactive peroxynitrite, which can nitrate tyrosines of proteins. The presence of nitrotyrosine is indicative of NO-induced 'oxidative stress'. Our data suggest that this mechanism may play a pathogenic role in IBM.

引用

页码：153 / 158

页数：6

共 25 条

[11] NITRIC-OXIDE REGULATES MITOCHONDRIAL RESPIRATION AND CELL FUNCTIONS BY INHIBITING CYTOCHROME-OXIDASE [J].

BROWN, GC .

FEBS LETTERS, 1995, 369 (2-3) :136-139

[12]

Crow J P, 1995, Curr Top Microbiol Immunol, V196, P57

[13]

DAWSON TM, 1994, J NEUROSCI, V14, P5147

[14]

ENGEL WK, 1967, PEDIATR CLIN N AM, V14, P963

[15] NITRIC-OXIDE SYNTHASE ISOZYMES - CHARACTERIZATION, PURIFICATION, MOLECULAR-CLONING, AND FUNCTIONS [J].

FORSTERMANN, U ;

CLOSS, EI ;

POLLOCK, JS ;

NAKANE, M ;

SCHWARZ, P ;

GATH, I ;

KLEINERT, H .

HYPERTENSION, 1994, 23 (06) :1121-1131

[16]

Good PF, 1996, AM J PATHOL, V149, P21

[17] BETA-AMYLOID 25-35 ACTIVATES NITRIC-OXIDE SYNTHASE IN A NEURONAL CLONE [J].

HU, JR ;

ELFAKAHANY, EE .

NEUROREPORT, 1993, 4 (06) :760-762

[18] Nitric oxide synthase and neuronal vulnerability in Parkinson's disease [J].

Hunot, S ;

Boissiere, F ;

Faucheux, B ;

Brugg, B ;

MouattPrigent, A ;

Agid, Y ;

Hirsch, EC .

NEUROSCIENCE, 1996, 72 (02) :355-363

[19] Difference in expression of phosphorylated tau epitopes between sporadic inclusion-body myositis and hereditary inclusion-body myopathies [J].

Mirabella, M ;

Alvarez, RB ;

Bilak, M ;

Engel, WK ;

Askanas, V .

JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY, 1996, 55 (07) :774-786

[20] CLONED HUMAN BRAIN NITRIC-OXIDE SYNTHASE IS HIGHLY EXPRESSED IN SKELETAL-MUSCLE [J].

NAKANE, M ;

SCHMIDT, HHHW ;

POLLOCK, JS ;

FORSTERMANN, U ;

MURAD, F .

FEBS LETTERS, 1993, 316 (02) :175-180

← 1 2 3 →