Application of immobilized enzyme reactor in on-line high performance liquid chromatography: A review

被引：135

作者：

Girelli, AM ^{[1
]}

Mattei, E ^{[1
]}

机构：

[1] Univ Roma La Sapienza, Dipartimento Chim, I-00185 Rome, Italy

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2005年 / 819卷 / 01期

关键词：

immobilized enzyme reactor; HPLC; chromatographic supports;

D O I：

10.1016/j.jchromb.2005.01.031

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This review summarizes all the research efforts in the last decade (1994-2003) that have been spent to the various application of immobilized enzyme reactor (IMER) in on-line high performance liquid chromatography (HPLC). All immobilization procedures including supports, kind of assembly into chromatographic system and methods are described. The effect of immobilization on enzymatic properties and stability of biocatalysts is considered. A brief survey of the main applications of IMER both as pre-column, post-column or column in the chemical, pharmaceutical, clinical and commodities fields is also reported. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：3 / 16

页数：14

共 112 条

[1]

AMANKWA LN, 1995, PROTEIN SCI, V4, P113

[2] Determination of inhibitors' potency (IC50) by a direct high-performance liquid chromatographic method on an immobilised acetylcholinesterase column [J].

Andrisano, V ;

Bartolini, M ;

Gotti, R ;

Cavrini, V ;

Felix, G .

JOURNAL OF CHROMATOGRAPHY B, 2001, 753 (02) :375-383

[3]

Arroyo M., 1998, ARS PHARM, V39, P23

[4] Monolithic micro-immobilized-enzyme reactor with human recombinant acetylcholinesterase for on-line inhibition studies [J].

Bartolini, M ;

Cavrini, V ;

Andrisano, V .

JOURNAL OF CHROMATOGRAPHY A, 2004, 1031 (1-2) :27-34

[5] Development and characterization of an immobilized enzyme reactor based on glyceraldehyde-3-phosphate dehydrogenase for on-line enzymatic studies [J].

Bartolini, M ;

Andrisano, V ;

Wainer, IW .

JOURNAL OF CHROMATOGRAPHY A, 2003, 987 (1-2) :331-340

[6] Drug affinity to immobilized target bio-polymers by high-performance liquid chromatography and capillary electrophoresis [J].

Bertucci, C ;

Bartolini, M ;

Gotti, R ;

Andrisano, V .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2003, 797 (1-2) :111-129

[7] Lipase-based HPLC stationary phase: enantioselective synthesis of 2-substituted 1,3-propanediol monoacetates [J].

Bertucci, C ;

Petri, A ;

Felix, G ;

Perini, B ;

Salvadori, P .

TETRAHEDRON-ASYMMETRY, 1999, 10 (22) :4455-4462

[8]

Bielavská M, 2003, CHEM LISTY, V97, P1190

[9] Activity behavior of a HPLC column including α-chymotrypsin immobilized monosized-porous particles [J].

Bilici, Z ;

Camli, ST ;

Unsal, E ;

Tuncel, A .

ANALYTICA CHIMICA ACTA, 2004, 516 (1-2) :125-133

[10] A REVIEW OF REACTION DETECTION IN HPLC [J].

BRINKMAN, UAT .

CHROMATOGRAPHIA, 1987, 24 :190-200

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