Application of immobilized enzyme reactor in on-line high performance liquid chromatography: A review

被引：135

作者：

Girelli, AM ^{[1
]}

Mattei, E ^{[1
]}

机构：

[1] Univ Roma La Sapienza, Dipartimento Chim, I-00185 Rome, Italy

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2005年 / 819卷 / 01期

关键词：

immobilized enzyme reactor; HPLC; chromatographic supports;

D O I：

10.1016/j.jchromb.2005.01.031

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This review summarizes all the research efforts in the last decade (1994-2003) that have been spent to the various application of immobilized enzyme reactor (IMER) in on-line high performance liquid chromatography (HPLC). All immobilization procedures including supports, kind of assembly into chromatographic system and methods are described. The effect of immobilization on enzymatic properties and stability of biocatalysts is considered. A brief survey of the main applications of IMER both as pre-column, post-column or column in the chemical, pharmaceutical, clinical and commodities fields is also reported. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：3 / 16

页数：14

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