Mutation (G275E) of the nicotinic acetylcholine receptor α6 subunit is associated with high levels of resistance to spinosyns in Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)

被引:75
作者
Silva, Wellington M. [1 ]
Berger, Madeleine [2 ]
Bass, Chris [3 ]
Williamson, Martin [2 ]
Moura, Danielle M. N. [4 ]
Ribeiro, Lilian M. S. [1 ]
Siqueira, Herbert A. A. [1 ]
机构
[1] Univ Fed Rural Pernambuco, Dept Agron Entomol, Rua Dom Manoel de Medeiros S-N, BR-52171900 Recife, PE, Brazil
[2] Rothamsted Res, Dept Biol Chem & Crop Protect, Harpenden AL5 2JQ, Herts, England
[3] Univ Exeter, Coll Life & Environm Sci, Biosci, Penryn Campus, Penryn TR10 9FE, Cornwall, England
[4] Aggeu Magalhaes FIOCRUZ, Dept Microbiol, BR-50732970 Recife, PE, Brazil
基金
英国生物技术与生命科学研究理事会;
关键词
Tomato leafminer; Insecticide resistance; nACh receptor; Target-site alteration; Metabolism; DROSOPHILA-MELANOGASTER; BRAZILIAN POPULATIONS; PYRETHROID RESISTANCE; DIAMONDBACK MOTH; SUSCEPTIBILITY; IDENTIFICATION; TRANSCRIPTS; MECHANISMS; D-ALPHA-6; SYNERGISM;
D O I
10.1016/j.pestbp.2016.02.006
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The tomato leafminer, Tuta absoluta, now a major pest of tomato crops worldwide, is primarily controlled using chemical insecticides. Recently, high levels of resistance to the insecticide spinosad have been described in T. absoluta populations in Brazil. Selection of a resistant field-collected strain led to very high levels of resistance to spinosad and cross-resistance to spinetoram, but not to other insecticides that target the nicotinic acetylcholine receptor (nAChR). In this study the mechanisms underlying resistance to spinosad were investigated using toxicological, biochemical and molecular approaches. Inhibition of metabolic enzymes using synergists and biochemical assessment of detoxification enzyme activity provided little evidence of metabolic resistance in the selected strain. Cloning and sequencing of the nAChR alpha 6 subunit from T. absoluta, the spinosad target-site, from susceptible and spinosad-resistant strains were done to investigate the role of a target-site mechanism in resistance. A single nucleotide change was identified in exon 9 of the a6 subunit of the resistant strain, resulting in the replacement of the glycine (G) residue at position 275 observed in susceptible T. absoluta strains with a glutamic acid (E). A high-throughput DNA-based diagnostic assay was developed and used to assess the prevalence of the G275E mutation in 17 field populations collected from different geographical regions of Brazil. The resistant allele was found at low frequency, and in the heterozygous form, in seven of these populations but at much higher frequency and in the homozygous form in a population collected in the Iraquara municipality. The frequency of the mutation was significantly correlated with the mortality of these populations in discriminating dose bioassays. In summary our results provide evidence that the G275E mutation is an important mechanism of resistance to spinosyns in T. absoluta, and may be used as a marker for resistance monitoring in field populations. (C) 2016 Elsevier Inc. All rights reserved.
引用
收藏
页码:1 / 8
页数:8
相关论文
共 56 条
[21]   Glutathione transferases [J].
Hayes, JD ;
Flanagan, JU ;
Jowsey, IR .
ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY, 2005, 45 :51-88
[22]   Hydrolysis of pyrethroids by carboxylesterases from Lucilia cuprina and Drosophila melanogaster with active sites modified by in vitro mutagenesis [J].
Heidari, R ;
Devonshire, AL ;
Campbell, BE ;
Dorrian, SJ ;
Oakeshott, JG ;
Russell, RJ .
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2005, 35 (06) :597-609
[23]   Truncated transcripts of nicotinic acetylcholine subunit gene Bdα6 are associated with spinosad resistance in Bactrocera dorsalis [J].
Hsu, Ju-Chun ;
Feng, Hai-Tung ;
Wu, Wen-Jer ;
Geib, Scott M. ;
Mao, Ching-hua ;
Vontas, John .
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2012, 42 (10) :806-815
[24]   The nicotinic acetylcholine receptor gene family of the honey bee, Apis mellifera [J].
Jones, Andrew K. ;
Raymond-Delpech, Valerie ;
Thany, Steeve H. ;
Gauthier, Monique ;
Sattelle, David B. .
GENOME RESEARCH, 2006, 16 (11) :1422-1430
[25]  
LeOra-Software, 2005, POLO PLUS POLO WIND
[26]   Insecticide resistance in Argentine populations of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) [J].
Lietti, MMM ;
Botto, E ;
Alzogaray, RA .
NEOTROPICAL ENTOMOLOGY, 2005, 34 (01) :113-119
[27]   Insecticide resistance and cross-resistance in the house fly (Diptera: Muscidae) [J].
Liu, NN ;
Yue, X .
JOURNAL OF ECONOMIC ENTOMOLOGY, 2000, 93 (04) :1269-1275
[28]   A Dα6 knockout strain of Drosophila melahogaster confers a high level of resistance to spinosad [J].
Perry, Trent ;
McKenzie, John A. ;
Batterham, Philip .
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2007, 37 (02) :184-188
[29]  
Picanco M., 1995, Horticultura Brasileira, V13, P180
[30]   A nicotinic acetylcholine receptor transmembrane point mutation (G275E) associated with resistance to spinosad in Frankliniella occidentalis [J].
Puinean, Alin M. ;
Lansdell, Stuart J. ;
Collins, Toby ;
Bielza, Pablo ;
Millar, Neil S. .
JOURNAL OF NEUROCHEMISTRY, 2013, 124 (05) :590-601